Differentiation and CRISPR-Cas9-mediated genetic engineering of human intestinal organoids

Differentiation and CRISPR-Cas9-mediated genetic engineering of human intestinal organoids

Summary: Intestinal organoids are three-dimensional cultures that resemble key aspects of the epithelium of origin. Here, we describe how to differentiate human small intestinal organoids by combining growth media variations and genetic engineering. We detail the differentiation of human intestinal...

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Bibliographic Details
Main Authors: Adriana Martinez-Silgado, Fjodor A. Yousef Yengej, Jens Puschhof, Veerle Geurts, Charelle Boot, Maarten H. Geurts, Maarten B. Rookmaaker, Marianne C. Verhaar, Joep Beumer, Hans Clevers
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
CRISPR
Cell differentiation
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722005196

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http://www.sciencedirect.com/science/article/pii/S2666166722005196

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