Development of a Methodology to Adapt an Equilibrium Buffer/Wash Applied to the Purification of hGPN2 Protein Expressed in <i>Escherichia coli</i> Using an IMAC Immobilized Metal Affinity Chromatography System
Protein purification is a complex and non-standardized process; the fact that proteins have different structural types making it difficult to create a standard methodology to obtain them in a pure, soluble, and homogeneous form. The present study shows the selective development of a buffer suitable...
| Egile Nagusiak: | , , , , |
|---|---|
| Formatua: | Artikulua |
| Hizkuntza: | English |
| Argitaratua: |
MDPI AG
2022-06-01
|
| Saila: | Separations |
| Gaiak: | |
| Sarrera elektronikoa: | https://www.mdpi.com/2297-8739/9/7/164 |