A Fast, Efficient and Easy to Implement Method to Purify Bacterial Pili From Lacticaseibacillus rhamnosus GG Based on Multimodal Chromatography

A Fast, Efficient and Easy to Implement Method to Purify Bacterial Pili From Lacticaseibacillus rhamnosus GG Based on Multimodal Chromatography

Pili are polymeric proteins located at the cell surface of bacteria. These filamentous proteins play a pivotal role in bacterial adhesion with the surrounding environment. They are found both in Gram-negative and Gram-positive bacteria but differ in their structural organization. Purifying these hig...

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Bibliographic Details
Main Authors: Raphael Dos Santos Morais, Sofiane El-Kirat-Chatel, Jennifer Burgain, Blandine Simard, Sarah Barrau, Cédric Paris, Frédéric Borges, Claire Gaiani
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Microbiology
Subjects:
Lacticaseibacillus rhamnosus GG
SpaCBA pili
purification
multimodal chromatography
light scattering
atomic force microscopy
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2020.609880/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2020.609880/full

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