Contamination of Citrobacter spp. in food and sales environment samples and the antibiotics resistance profile of the isolates
Objective To investigate the contamination of Citrobacter spp. in food and sales environment samples in Shijiazhuang City discovery the antibiotics resistance profile of the Citrobacter spp. isolates, and provide scientific basis for the prevention of foodborne diseases caused by the bacterium and c...
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2021-03-01
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| Series: | Zhongguo shipin weisheng zazhi |
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| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/20210202 |
| _version_ | 1797953981297721344 |
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| author | Liyun QIN Ruiping JIANG Weili GAO Guoping LYU Xinchao LYU Xin SU |
| author_facet | Liyun QIN Ruiping JIANG Weili GAO Guoping LYU Xinchao LYU Xin SU |
| author_sort | Liyun QIN |
| collection | DOAJ |
| description | Objective To investigate the contamination of Citrobacter spp. in food and sales environment samples in Shijiazhuang City discovery the antibiotics resistance profile of the Citrobacter spp. isolates, and provide scientific basis for the prevention of foodborne diseases caused by the bacterium and clinical treatment. Methods Four kinds of food samples including animal meat, poultry meat, aquatic products and cooked food and sales environment samples were collected from four urban counties and four suburban counties in Shijiazhuang. Citrobacter spp. were isolated and then the antibiotics resistance analysis of the isolates were performed. All the strains were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS) system and VITEK 2 Compact system. Antibiotics susceptibility was performed by broth microdilution method. Results Among the 320 samples, 62 samples were Citrobacter spp. positive(19.4%,62/320), and 72 Citrobacter spp. strains were isolated. The contamination rate of Citrobacter spp. were 28.2%(33/117) in animal meat, 21.3%(10/47) in poultry meat, 18.2%(8/44) in aquatic products, 15.7%(8/51) in cooked food and 4.9%(3/61) in sales environment samples. There were significant differences in the contamination rate of Citrobacter spp. in food samples and sales environment samples (χ2=10.08, P<0.01) , but the differences among four kinds of food samples were not significant (χ2=4.01, P>0.05). The antibiotic resistance of the strains were serious. Most of the Citrobacter spp. strains were resistant to cefazolin(93.1%, 67/72), and cefoxitin(81.9%, 59/72). All of the Citrobacter spp. strains were not sensitive to meropenem. There were seven Citrobacter spp. produced extended-spectrum β- lactamase (ESBLs),, and most of the Citrobacter spp. strains were found to be multiple drug resistance(MDR)(91.7%, 66/72). Conclusion The contamination of Citrobacter spp. in food samples was quite severe and most of the Citrobacter spp. isolates were antibiotic resistant and MDR. Citrobacter spp. had a potential food poisoning risk and it should be paid great attention. |
| first_indexed | 2024-04-10T23:10:32Z |
| format | Article |
| id | doaj.art-dda4515a113b4bae99f7525750ea6f79 |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-04-10T23:10:32Z |
| publishDate | 2021-03-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-dda4515a113b4bae99f7525750ea6f792023-01-13T05:54:15ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562021-03-01330213113710.13590/j.cjfh.2021.02.0021004-8456(2021)02-0131-07Contamination of Citrobacter spp. in food and sales environment samples and the antibiotics resistance profile of the isolatesLiyun QIN0Ruiping JIANG1Weili GAO2Guoping LYU3Xinchao LYU4Xin SU5Key Laboratory for Unidentificial Bacteria Research of Hebei Province, Shijiazhuang Center for Disease Control and Prevention, Hebei Shijiazhuang 050011, ChinaKey Laboratory for Unidentificial Bacteria Research of Hebei Province, Shijiazhuang Center for Disease Control and Prevention, Hebei Shijiazhuang 050011, ChinaKey Laboratory for Unidentificial Bacteria Research of Hebei Province, Shijiazhuang Center for Disease Control and Prevention, Hebei Shijiazhuang 050011, ChinaHebei University of Chinese Medicine, Hebei Shijiazhuang 050000, ChinaKey Laboratory for Unidentificial Bacteria Research of Hebei Province, Shijiazhuang Center for Disease Control and Prevention, Hebei Shijiazhuang 050011, ChinaKey Laboratory for Unidentificial Bacteria Research of Hebei Province, Shijiazhuang Center for Disease Control and Prevention, Hebei Shijiazhuang 050011, ChinaObjective To investigate the contamination of Citrobacter spp. in food and sales environment samples in Shijiazhuang City discovery the antibiotics resistance profile of the Citrobacter spp. isolates, and provide scientific basis for the prevention of foodborne diseases caused by the bacterium and clinical treatment. Methods Four kinds of food samples including animal meat, poultry meat, aquatic products and cooked food and sales environment samples were collected from four urban counties and four suburban counties in Shijiazhuang. Citrobacter spp. were isolated and then the antibiotics resistance analysis of the isolates were performed. All the strains were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS) system and VITEK 2 Compact system. Antibiotics susceptibility was performed by broth microdilution method. Results Among the 320 samples, 62 samples were Citrobacter spp. positive(19.4%,62/320), and 72 Citrobacter spp. strains were isolated. The contamination rate of Citrobacter spp. were 28.2%(33/117) in animal meat, 21.3%(10/47) in poultry meat, 18.2%(8/44) in aquatic products, 15.7%(8/51) in cooked food and 4.9%(3/61) in sales environment samples. There were significant differences in the contamination rate of Citrobacter spp. in food samples and sales environment samples (χ2=10.08, P<0.01) , but the differences among four kinds of food samples were not significant (χ2=4.01, P>0.05). The antibiotic resistance of the strains were serious. Most of the Citrobacter spp. strains were resistant to cefazolin(93.1%, 67/72), and cefoxitin(81.9%, 59/72). All of the Citrobacter spp. strains were not sensitive to meropenem. There were seven Citrobacter spp. produced extended-spectrum β- lactamase (ESBLs),, and most of the Citrobacter spp. strains were found to be multiple drug resistance(MDR)(91.7%, 66/72). Conclusion The contamination of Citrobacter spp. in food samples was quite severe and most of the Citrobacter spp. isolates were antibiotic resistant and MDR. Citrobacter spp. had a potential food poisoning risk and it should be paid great attention.http://www.zgspws.com/zgspwszz/article/abstract/20210202citrobacter spp.foodsales environmentcontaminationantibiotics resistancefoodborne disease |
| spellingShingle | Liyun QIN Ruiping JIANG Weili GAO Guoping LYU Xinchao LYU Xin SU Contamination of Citrobacter spp. in food and sales environment samples and the antibiotics resistance profile of the isolates Zhongguo shipin weisheng zazhi citrobacter spp. food sales environment contamination antibiotics resistance foodborne disease |
| title | Contamination of Citrobacter spp. in food and sales environment samples and the antibiotics resistance profile of the isolates |
| title_full | Contamination of Citrobacter spp. in food and sales environment samples and the antibiotics resistance profile of the isolates |
| title_fullStr | Contamination of Citrobacter spp. in food and sales environment samples and the antibiotics resistance profile of the isolates |
| title_full_unstemmed | Contamination of Citrobacter spp. in food and sales environment samples and the antibiotics resistance profile of the isolates |
| title_short | Contamination of Citrobacter spp. in food and sales environment samples and the antibiotics resistance profile of the isolates |
| title_sort | contamination of citrobacter spp in food and sales environment samples and the antibiotics resistance profile of the isolates |
| topic | citrobacter spp. food sales environment contamination antibiotics resistance foodborne disease |
| url | http://www.zgspws.com/zgspwszz/article/abstract/20210202 |
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