Isolating peripheral blood mononuclear cells from HIV-infected patients for single-cell RNA sequencing and integration analysis

Isolating peripheral blood mononuclear cells from HIV-infected patients for single-cell RNA sequencing and integration analysis

Summary: Single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) allows the dissection of transcriptional changes in immune cells with HIV infection. Here, we isolate PBMCs from HIV-infected individuals. After counting the cell number and verifying cell viability, we per...

Full description

Bibliographic Details
Main Authors: Jie Jia, Yu Zhao, Jian-Hua Wang, Yi-Qun Kuang
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Bioinformatics
Cell Biology
Cell Isolation
Cell-based Assays
Immunology
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001806

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723001806

Similar Items