Role of hyperbaric oxygen therapy in PDGF-BB-mediated astrogliosis in traumatic brain injury rats associated with ERK1/2 signaling pathway inhibition
Abstract Background Hyperbaric oxygen (HBO) plays positive roles in the therapy of traumatic brain injury (TBI); however, the mechanism underlying its effects on TBI is largely unknown. The study aims to elucidate the molecular mechanism implicated with the interaction between platelet-derived growt...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-02-01
|
| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40001-023-01062-1 |
| _version_ | 1797865223558791168 |
|---|---|
| author | Guanghui Xiu Xiuling Li Qiang Li Yunyu Yin Qiqi Tang Jintao Li Jiaying Ling Bin Ling Ying Yang |
| author_facet | Guanghui Xiu Xiuling Li Qiang Li Yunyu Yin Qiqi Tang Jintao Li Jiaying Ling Bin Ling Ying Yang |
| author_sort | Guanghui Xiu |
| collection | DOAJ |
| description | Abstract Background Hyperbaric oxygen (HBO) plays positive roles in the therapy of traumatic brain injury (TBI); however, the mechanism underlying its effects on TBI is largely unknown. The study aims to elucidate the molecular mechanism implicated with the interaction between platelet-derived growth factor-BB (PDGF-BB) and extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway, which may play critical roles during HBO treatment both in the astrocyte scratching model in vitro and rat TBI model in vivo. Methods Changes in neurological function and wound healing were evaluated using the neurological severity scores (NSS) scale, immunohistochemistry, western blotting, and qRT-PCR, respectively. Results The results showed that PDGF-BBi (PDGB interfered with small RNA) dramatically improves neuronal viability in vitro when transfected into the scratched astrocytes derived from the cerebral cortex of neonatal rats. Moreover, in vivo experiments revealed that HBO therapy substantially elevated the NSS scores and simultaneously reduced the mortality in TBI rats, as indicated by the NSS scales. Notably, HBO therapy was found to possess the ability to inhibit glial cell proliferation, promote the regeneration of neurons and synapses, and ultimately facilitate the wound healing, as revealed by immunohistochemistry and glial scar formation found in TBI rats. Importantly, HBO markedly decreased the expression levels of PDGF-BB and ERK1/2. It can clearly be seen that downregulated PDGF-BB and ERK1/2 levels were corresponding with the status of significant amelioration of the therapeutic effect of HBO. Conversely, the upregulation of PDGF-BB and ERK1/2 levels was in line with the opposite effect. Conclusion It has been concluded that HBO therapy may play its active role in TBI treatment dependent on astrogliosis inhibition, which may be achieved by downregulating the ERK1/2 signaling pathway mediated by PDGF-BB. |
| first_indexed | 2024-04-09T23:05:44Z |
| format | Article |
| id | doaj.art-ddad8441a8f24233bc46c3f7b7a2bba0 |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-04-09T23:05:44Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-ddad8441a8f24233bc46c3f7b7a2bba02023-03-22T10:46:19ZengBMCEuropean Journal of Medical Research2047-783X2023-02-0128111310.1186/s40001-023-01062-1Role of hyperbaric oxygen therapy in PDGF-BB-mediated astrogliosis in traumatic brain injury rats associated with ERK1/2 signaling pathway inhibitionGuanghui Xiu0Xiuling Li1Qiang Li2Yunyu Yin3Qiqi Tang4Jintao Li5Jiaying Ling6Bin Ling7Ying Yang8Affiliated Hospital of Yunnan University, School of Medicine, Yunnan UniversityDepartment of Obstetrics, The First People’s Hospital of Yunnan ProvinceDepartment of Emergency Medicine, Fushun People’s HospitalDepartment of Intensive Care Unit, Affiliated Hospital of North Sichuan Medical CollegeAffiliated Hospital of Yunnan University, School of Medicine, Yunnan UniversityInstitute of Neuroscience, Kunming Medicine UniversityKunming Medical University Haiyuan CollegeAffiliated Hospital of Yunnan University, School of Medicine, Yunnan UniversityAffiliated Hospital of Yunnan University, School of Medicine, Yunnan UniversityAbstract Background Hyperbaric oxygen (HBO) plays positive roles in the therapy of traumatic brain injury (TBI); however, the mechanism underlying its effects on TBI is largely unknown. The study aims to elucidate the molecular mechanism implicated with the interaction between platelet-derived growth factor-BB (PDGF-BB) and extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway, which may play critical roles during HBO treatment both in the astrocyte scratching model in vitro and rat TBI model in vivo. Methods Changes in neurological function and wound healing were evaluated using the neurological severity scores (NSS) scale, immunohistochemistry, western blotting, and qRT-PCR, respectively. Results The results showed that PDGF-BBi (PDGB interfered with small RNA) dramatically improves neuronal viability in vitro when transfected into the scratched astrocytes derived from the cerebral cortex of neonatal rats. Moreover, in vivo experiments revealed that HBO therapy substantially elevated the NSS scores and simultaneously reduced the mortality in TBI rats, as indicated by the NSS scales. Notably, HBO therapy was found to possess the ability to inhibit glial cell proliferation, promote the regeneration of neurons and synapses, and ultimately facilitate the wound healing, as revealed by immunohistochemistry and glial scar formation found in TBI rats. Importantly, HBO markedly decreased the expression levels of PDGF-BB and ERK1/2. It can clearly be seen that downregulated PDGF-BB and ERK1/2 levels were corresponding with the status of significant amelioration of the therapeutic effect of HBO. Conversely, the upregulation of PDGF-BB and ERK1/2 levels was in line with the opposite effect. Conclusion It has been concluded that HBO therapy may play its active role in TBI treatment dependent on astrogliosis inhibition, which may be achieved by downregulating the ERK1/2 signaling pathway mediated by PDGF-BB.https://doi.org/10.1186/s40001-023-01062-1Hyperbaric oxygen therapyTraumatic brain injuryPlatelet-derived growth factorExtracellular signal-regulated kinase 1/2AstrogliosisNeural regeneration |
| spellingShingle | Guanghui Xiu Xiuling Li Qiang Li Yunyu Yin Qiqi Tang Jintao Li Jiaying Ling Bin Ling Ying Yang Role of hyperbaric oxygen therapy in PDGF-BB-mediated astrogliosis in traumatic brain injury rats associated with ERK1/2 signaling pathway inhibition European Journal of Medical Research Hyperbaric oxygen therapy Traumatic brain injury Platelet-derived growth factor Extracellular signal-regulated kinase 1/2 Astrogliosis Neural regeneration |
| title | Role of hyperbaric oxygen therapy in PDGF-BB-mediated astrogliosis in traumatic brain injury rats associated with ERK1/2 signaling pathway inhibition |
| title_full | Role of hyperbaric oxygen therapy in PDGF-BB-mediated astrogliosis in traumatic brain injury rats associated with ERK1/2 signaling pathway inhibition |
| title_fullStr | Role of hyperbaric oxygen therapy in PDGF-BB-mediated astrogliosis in traumatic brain injury rats associated with ERK1/2 signaling pathway inhibition |
| title_full_unstemmed | Role of hyperbaric oxygen therapy in PDGF-BB-mediated astrogliosis in traumatic brain injury rats associated with ERK1/2 signaling pathway inhibition |
| title_short | Role of hyperbaric oxygen therapy in PDGF-BB-mediated astrogliosis in traumatic brain injury rats associated with ERK1/2 signaling pathway inhibition |
| title_sort | role of hyperbaric oxygen therapy in pdgf bb mediated astrogliosis in traumatic brain injury rats associated with erk1 2 signaling pathway inhibition |
| topic | Hyperbaric oxygen therapy Traumatic brain injury Platelet-derived growth factor Extracellular signal-regulated kinase 1/2 Astrogliosis Neural regeneration |
| url | https://doi.org/10.1186/s40001-023-01062-1 |
| work_keys_str_mv | AT guanghuixiu roleofhyperbaricoxygentherapyinpdgfbbmediatedastrogliosisintraumaticbraininjuryratsassociatedwitherk12signalingpathwayinhibition AT xiulingli roleofhyperbaricoxygentherapyinpdgfbbmediatedastrogliosisintraumaticbraininjuryratsassociatedwitherk12signalingpathwayinhibition AT qiangli roleofhyperbaricoxygentherapyinpdgfbbmediatedastrogliosisintraumaticbraininjuryratsassociatedwitherk12signalingpathwayinhibition AT yunyuyin roleofhyperbaricoxygentherapyinpdgfbbmediatedastrogliosisintraumaticbraininjuryratsassociatedwitherk12signalingpathwayinhibition AT qiqitang roleofhyperbaricoxygentherapyinpdgfbbmediatedastrogliosisintraumaticbraininjuryratsassociatedwitherk12signalingpathwayinhibition AT jintaoli roleofhyperbaricoxygentherapyinpdgfbbmediatedastrogliosisintraumaticbraininjuryratsassociatedwitherk12signalingpathwayinhibition AT jiayingling roleofhyperbaricoxygentherapyinpdgfbbmediatedastrogliosisintraumaticbraininjuryratsassociatedwitherk12signalingpathwayinhibition AT binling roleofhyperbaricoxygentherapyinpdgfbbmediatedastrogliosisintraumaticbraininjuryratsassociatedwitherk12signalingpathwayinhibition AT yingyang roleofhyperbaricoxygentherapyinpdgfbbmediatedastrogliosisintraumaticbraininjuryratsassociatedwitherk12signalingpathwayinhibition |