Successful gene expression analysis by multiplex, real-time, one-step RT-PCR, irrespective of the targets amplified
Multiplex, real-time, one-step RT-PCR enables simultaneous quantification of several transcripts in one reaction vessel. This saves time and conserves precious sample material when analyzing the expression of reference and target genes in a particular sample. However, intensive PCR optimization is o...
Principais autores: | , , , |
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Formato: | Artigo |
Idioma: | English |
Publicado em: |
Taylor & Francis Group
2007-08-01
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coleção: | BioTechniques |
Acesso em linha: | https://www.future-science.com/doi/10.2144/000112560 |