Successful gene expression analysis by multiplex, real-time, one-step RT-PCR, irrespective of the targets amplified

Multiplex, real-time, one-step RT-PCR enables simultaneous quantification of several transcripts in one reaction vessel. This saves time and conserves precious sample material when analyzing the expression of reference and target genes in a particular sample. However, intensive PCR optimization is o...

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Detalhes bibliográficos
Principais autores: Holger Engel, Corinna Kueppers, Margaretha Koenig, Dirk Loeffert
Formato: Artigo
Idioma:English
Publicado em: Taylor & Francis Group 2007-08-01
coleção:BioTechniques
Acesso em linha:https://www.future-science.com/doi/10.2144/000112560