Evaluation of the Structure–Function Relationship of SGNH Lipase from <i>Streptomyces rimosus</i> by Site-Directed Mutagenesis and Computational Approach

Evaluation of the Structure–Function Relationship of SGNH Lipase from <i>Streptomyces rimosus</i> by Site-Directed Mutagenesis and Computational Approach

<i>Streptomyces rimosus</i> extracellular lipase (SrL) is a multifunctional hydrolase belonging to the SGNH family. Here site-directed mutagenesis (SDM) was used for the first time to investigate the functional significance of the conserved amino acid residues Ser10, Gly54, Asn82, Asn213...

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Bibliographic Details
Main Authors: Želimira Filić, Ana Bielen, Ela Šarić, Mirsada Ćehić, Ivo Crnolatac, Sanja Tomić, Dušica Vujaklija, Marija Abramić
Format: Article
Language:English
Published: MDPI AG 2024-01-01
Series:International Journal of Molecular Sciences
Subjects:
SGNH/GDSL-hydrolase
site-directed mutagenesis
catalytic residues
molecular docking
molecular dynamics
protein–substrate interactions
Online Access:https://www.mdpi.com/1422-0067/25/1/595

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https://www.mdpi.com/1422-0067/25/1/595

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