A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens
With the improvement of people’s living standards, food safety has attracted more and more social attention. Organisms, especially microorganisms, are the most important factor that affects food safety. There are many methods currently available to detect biological (microbial) factors in the field...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-05-01
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| Series: | Shipin Kexue |
| Subjects: | |
| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-9-035.pdf |
| _version_ | 1797805589568421888 |
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| author | WANG Shuai, YANG Yange, WU Zhanwen, LI Hongna, LI Tao, SUN Dongmei, YUAN Fei |
| author_facet | WANG Shuai, YANG Yange, WU Zhanwen, LI Hongna, LI Tao, SUN Dongmei, YUAN Fei |
| author_sort | WANG Shuai, YANG Yange, WU Zhanwen, LI Hongna, LI Tao, SUN Dongmei, YUAN Fei |
| collection | DOAJ |
| description | With the improvement of people’s living standards, food safety has attracted more and more social attention. Organisms, especially microorganisms, are the most important factor that affects food safety. There are many methods currently available to detect biological (microbial) factors in the field of food safety, including isothermal amplification technology especially the new techniques of recombinase polymerase amplification (RPA), recombinase-aided amplification (RAA) and enzymatic recombinase amplification (ERA), which is widely used for food safety detection because it can detect biological ingredients rapidly and accurately without relying on complicated instruments. The three isothermal amplification techniques require milder reaction conditions and simpler design of primers than others, despite having similar principles. In this article, we review the principles and concepts of these techniques and their recent application in the rapid detection of food pathogens, summarize their advantages and problems in food inspection, and present future prospects. |
| first_indexed | 2024-03-13T05:54:20Z |
| format | Article |
| id | doaj.art-dde426a7a88a44a5be9a898b376788b0 |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-13T05:54:20Z |
| publishDate | 2023-05-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-dde426a7a88a44a5be9a898b376788b02023-06-13T07:42:08ZengChina Food Publishing CompanyShipin Kexue1002-66302023-05-0144929730510.7506/spkx1002-6630-20220526-322A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne PathogensWANG Shuai, YANG Yange, WU Zhanwen, LI Hongna, LI Tao, SUN Dongmei, YUAN Fei0(1. College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163000, China; 2. Key Laboratory of Food Quality and Safety for State Market Regulation, Chinese Academy of Inspection and Quarantine, Beijing 100176, China)With the improvement of people’s living standards, food safety has attracted more and more social attention. Organisms, especially microorganisms, are the most important factor that affects food safety. There are many methods currently available to detect biological (microbial) factors in the field of food safety, including isothermal amplification technology especially the new techniques of recombinase polymerase amplification (RPA), recombinase-aided amplification (RAA) and enzymatic recombinase amplification (ERA), which is widely used for food safety detection because it can detect biological ingredients rapidly and accurately without relying on complicated instruments. The three isothermal amplification techniques require milder reaction conditions and simpler design of primers than others, despite having similar principles. In this article, we review the principles and concepts of these techniques and their recent application in the rapid detection of food pathogens, summarize their advantages and problems in food inspection, and present future prospects.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-9-035.pdfrecombinase polymerase amplification; recombinase-aided amplification; enzymatic recombinase amplification; foodborne pathogens; rapid detection |
| spellingShingle | WANG Shuai, YANG Yange, WU Zhanwen, LI Hongna, LI Tao, SUN Dongmei, YUAN Fei A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens Shipin Kexue recombinase polymerase amplification; recombinase-aided amplification; enzymatic recombinase amplification; foodborne pathogens; rapid detection |
| title | A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens |
| title_full | A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens |
| title_fullStr | A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens |
| title_full_unstemmed | A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens |
| title_short | A Review of the Application of Recombinase Polymerase Amplification, Recombinase-Aided Amplification and Enzymatic Recombinase Amplification in Rapid Detection of Foodborne Pathogens |
| title_sort | review of the application of recombinase polymerase amplification recombinase aided amplification and enzymatic recombinase amplification in rapid detection of foodborne pathogens |
| topic | recombinase polymerase amplification; recombinase-aided amplification; enzymatic recombinase amplification; foodborne pathogens; rapid detection |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-9-035.pdf |
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