The Determination of Soil Microbial Biomass Carbon and Adenosine Triphosphate Concentrations at Different Temperatures
Freezing soil samples at subzero temperatures is a commonly employed preservation method in soil science to halt microbial activity and enzymatic processes, preserving the sample’s composition and structure. The concentration of adenosine triphosphate (ATP) and the soil microbial biomass carbon cont...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Engineering Proceedings |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-4591/57/1/31 |
| _version_ | 1827306382997585920 |
|---|---|
| author | Lenka Bobuľská Lenka Demková Tomáš Lošák |
| author_facet | Lenka Bobuľská Lenka Demková Tomáš Lošák |
| author_sort | Lenka Bobuľská |
| collection | DOAJ |
| description | Freezing soil samples at subzero temperatures is a commonly employed preservation method in soil science to halt microbial activity and enzymatic processes, preserving the sample’s composition and structure. The concentration of adenosine triphosphate (ATP) and the soil microbial biomass carbon content were determined from soil samples stored at +4 °C, −21 °C and −80 °C for 24 h, 7 days, and 20 days. The results showed that the ATP in the soil was not significantly affected by temperature or storage time. Larger differences were observed in the carbon content of microbial biomass, where the amount of this parameter increased by 14.2% after 20 days of the experiment. |
| first_indexed | 2024-04-24T18:20:43Z |
| format | Article |
| id | doaj.art-ddf3da05ccc84521933a9b622b4f127d |
| institution | Directory Open Access Journal |
| issn | 2673-4591 |
| language | English |
| last_indexed | 2024-04-24T18:20:43Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Engineering Proceedings |
| spelling | doaj.art-ddf3da05ccc84521933a9b622b4f127d2024-03-27T13:36:18ZengMDPI AGEngineering Proceedings2673-45912023-12-015713110.3390/engproc2023057031The Determination of Soil Microbial Biomass Carbon and Adenosine Triphosphate Concentrations at Different TemperaturesLenka Bobuľská0Lenka Demková1Tomáš Lošák2Department of Ecology, Faculty of Humanities and Natural Sciences, University of Prešov, 17. November 1, 080 01 Prešov, SlovakiaDepartment of Ecology, Faculty of Humanities and Natural Sciences, University of Prešov, 17. November 1, 080 01 Prešov, SlovakiaDepartment of Environmentalistics and Natural Resources, Faculty of Regional Development and Interna-tional Studies, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech RepublicFreezing soil samples at subzero temperatures is a commonly employed preservation method in soil science to halt microbial activity and enzymatic processes, preserving the sample’s composition and structure. The concentration of adenosine triphosphate (ATP) and the soil microbial biomass carbon content were determined from soil samples stored at +4 °C, −21 °C and −80 °C for 24 h, 7 days, and 20 days. The results showed that the ATP in the soil was not significantly affected by temperature or storage time. Larger differences were observed in the carbon content of microbial biomass, where the amount of this parameter increased by 14.2% after 20 days of the experiment.https://www.mdpi.com/2673-4591/57/1/31soil freezingmicrobial activityenzymessoil storage |
| spellingShingle | Lenka Bobuľská Lenka Demková Tomáš Lošák The Determination of Soil Microbial Biomass Carbon and Adenosine Triphosphate Concentrations at Different Temperatures Engineering Proceedings soil freezing microbial activity enzymes soil storage |
| title | The Determination of Soil Microbial Biomass Carbon and Adenosine Triphosphate Concentrations at Different Temperatures |
| title_full | The Determination of Soil Microbial Biomass Carbon and Adenosine Triphosphate Concentrations at Different Temperatures |
| title_fullStr | The Determination of Soil Microbial Biomass Carbon and Adenosine Triphosphate Concentrations at Different Temperatures |
| title_full_unstemmed | The Determination of Soil Microbial Biomass Carbon and Adenosine Triphosphate Concentrations at Different Temperatures |
| title_short | The Determination of Soil Microbial Biomass Carbon and Adenosine Triphosphate Concentrations at Different Temperatures |
| title_sort | determination of soil microbial biomass carbon and adenosine triphosphate concentrations at different temperatures |
| topic | soil freezing microbial activity enzymes soil storage |
| url | https://www.mdpi.com/2673-4591/57/1/31 |
| work_keys_str_mv | AT lenkabobulska thedeterminationofsoilmicrobialbiomasscarbonandadenosinetriphosphateconcentrationsatdifferenttemperatures AT lenkademkova thedeterminationofsoilmicrobialbiomasscarbonandadenosinetriphosphateconcentrationsatdifferenttemperatures AT tomaslosak thedeterminationofsoilmicrobialbiomasscarbonandadenosinetriphosphateconcentrationsatdifferenttemperatures AT lenkabobulska determinationofsoilmicrobialbiomasscarbonandadenosinetriphosphateconcentrationsatdifferenttemperatures AT lenkademkova determinationofsoilmicrobialbiomasscarbonandadenosinetriphosphateconcentrationsatdifferenttemperatures AT tomaslosak determinationofsoilmicrobialbiomasscarbonandadenosinetriphosphateconcentrationsatdifferenttemperatures |