Alteration of sequence specificity of the type IIS restriction endonuclease BtsI.

Alteration of sequence specificity of the type IIS restriction endonuclease BtsI.

The Type IIS restriction endonuclease BtsI recognizes and digests at GCAGTG(2/0). It comprises two subunits: BtsIA and BtsIB. The BtsIB subunit contains the recognition domain, one catalytic domain for bottom strand nicking and part of the catalytic domain for the top strand nicking. BtsIA has the r...

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Bibliographic Details
Main Authors: Shengxi Guan, Aine Blanchard, Penghua Zhang, Zhenyu Zhu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2909958?pdf=render

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http://europepmc.org/articles/PMC2909958?pdf=render

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