Reduced off-target effect of NG-BE4max by using NG-HiFi system

Reduced off-target effect of NG-BE4max by using NG-HiFi system

Recently, a rationally engineered SpCas9 variant (SpCas9-NG) that can recognize a minimal NG protospacer adjacent motif (PAM) was reported to expand the targeting scope in genome editing. However, increased genome-wide off-target mutations with this variant compared with SpCas9 were reported in prev...

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Bibliographic Details
Main Authors: Huanhuan Shan, Zhiquan Liu, Yingqi Jia, Siyu Chen, Mao Chen, Yuning Song, Tingting Sui, Liangxue Lai, Zhanjun Li
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
SpCas9-NG
NG-BE4max
Hoxc13
off-target
NG-HiFi
rabbit
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253121001281

Internet

http://www.sciencedirect.com/science/article/pii/S2162253121001281

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