Metabolic labelling of a subpopulation of small extracellular vesicles using a fluorescent palmitic acid analogue

Metabolic labelling of a subpopulation of small extracellular vesicles using a fluorescent palmitic acid analogue

Abstract Exosomes are among the most puzzling vehicles of intercellular communication, but several crucial aspects of their biogenesis remain elusive, primarily due to the difficulty in purifying vesicles with similar sizes and densities. Here we report an effective methodology for labelling small e...

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Bibliographic Details
Main Authors: Valeria Barreca, Zaira Boussadia, Deborah Polignano, Lorenzo Galli, Valentina Tirelli, Massimo Sanchez, Mario Falchi, Lucia Bertuccini, Francesca Iosi, Massimo Tatti, Massimo Sargiacomo, Maria Luisa Fiani
Format: Article
Language:English
Published: Wiley 2023-12-01
Series:Journal of Extracellular Vesicles
Subjects:
exosome biogenesis
exosomes
extracellular vesicles
lipid metabolism
MVB
sEV
Online Access:https://doi.org/10.1002/jev2.12392

Internet

https://doi.org/10.1002/jev2.12392

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