Using Next-Generation Sequencing for DNA Barcoding: Capturing Allelic Variation in ITS2

Internal Transcribed Spacer 2 (ITS2) is a popular DNA barcoding marker; however, in some animal species it is hypervariable and therefore difficult to sequence with traditional methods. With next-generation sequencing (NGS) it is possible to sequence all gene variants despite the presence of single...

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Bibliographic Details
Main Authors: Jana Batovska, Noel O. I. Cogan, Stacey E. Lynch, Mark J. Blacket
Format: Article
Language:English
Published: Oxford University Press 2017-01-01
Series:G3: Genes, Genomes, Genetics
Subjects:
Online Access:http://g3journal.org/lookup/doi/10.1534/g3.116.036145