Using Next-Generation Sequencing for DNA Barcoding: Capturing Allelic Variation in ITS2
Internal Transcribed Spacer 2 (ITS2) is a popular DNA barcoding marker; however, in some animal species it is hypervariable and therefore difficult to sequence with traditional methods. With next-generation sequencing (NGS) it is possible to sequence all gene variants despite the presence of single...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Oxford University Press
2017-01-01
|
Series: | G3: Genes, Genomes, Genetics |
Subjects: | |
Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.116.036145 |