Electronic Circular Dichroism of the Cas9 Protein and gRNA:Cas9 Ribonucleoprotein Complex

Electronic Circular Dichroism of the Cas9 Protein and gRNA:Cas9 Ribonucleoprotein Complex

The <i>Streptococcus pyogenes</i> Cas9 protein (SpCas9), a component of CRISPR-based immune system in microbes, has become commonly utilized for genome editing. This nuclease forms a ribonucleoprotein (RNP) complex with guide RNA (gRNA) which induces Cas9 structural changes and triggers...

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Bibliographic Details
Main Authors: Monika Halat, Magdalena Klimek-Chodacka, Jagoda Orleanska, Malgorzata Baranska, Rafal Baranski
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:International Journal of Molecular Sciences
Subjects:
CRISPR/Cas9
DNA cleavage
ECD
spectroscopy
guide RNA
RNP complex
Online Access:https://www.mdpi.com/1422-0067/22/6/2937

Internet

https://www.mdpi.com/1422-0067/22/6/2937

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