| Summary: | The work aimed to improve the extraction efficiency of wheat bran polysaccharide by solid-state fermentation using the bioactive fungus <i>Isaria cicadae Miq</i>. and identify the structural properties of fermented wheat bran polysaccharide (IC-FWBP). The polysaccharide fraction of IC-FWBP was isolated with an extraction yield of 2.88% and an average molecular weight of 3.31 × 10<sup>6</sup> Da. The IC-FWBP was comprised of mannose, glucose, and galactose. The methylation, nuclear magnetic resonance (NMR), and Congo red analysis results suggested that IC-FWBP contained glycosidic linkages of T-<i>β</i>-D-Glc<i>f</i>, 1→2-<i>α</i>-D-Man<i>p</i>, 1→5,6-<i>β</i>-D-Gal<i>f</i>, 1→2,3,4-<i>α</i>-D-Gal<i>p</i> and 1→2,3,4-<i>β</i>-D-Man<i>p</i>, with triple-helix conformations. The morphological observation showed that IC-FWBP was composed of rod-like and spherical particles. These investigations on the structural properties of IC-FWBP will be beneficial to further research on the functional properties of wheat bran polysaccharides.
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