Structural Properties of a Heteropolysaccharide Released from <i>Isaria cicadae Miq.</i> Solid-State Fermented Wheat Bran
The work aimed to improve the extraction efficiency of wheat bran polysaccharide by solid-state fermentation using the bioactive fungus <i>Isaria cicadae Miq</i>. and identify the structural properties of fermented wheat bran polysaccharide (IC-FWBP). The polysaccharide fraction of IC-FW...
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MDPI AG
2023-03-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/3/309 |
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| author | Rui Liu Ruixin Liu Xuebing Yan Ningjie Li Ming Li Zijian Zhi Boli Guo Min Zhang |
| author_facet | Rui Liu Ruixin Liu Xuebing Yan Ningjie Li Ming Li Zijian Zhi Boli Guo Min Zhang |
| author_sort | Rui Liu |
| collection | DOAJ |
| description | The work aimed to improve the extraction efficiency of wheat bran polysaccharide by solid-state fermentation using the bioactive fungus <i>Isaria cicadae Miq</i>. and identify the structural properties of fermented wheat bran polysaccharide (IC-FWBP). The polysaccharide fraction of IC-FWBP was isolated with an extraction yield of 2.88% and an average molecular weight of 3.31 × 10<sup>6</sup> Da. The IC-FWBP was comprised of mannose, glucose, and galactose. The methylation, nuclear magnetic resonance (NMR), and Congo red analysis results suggested that IC-FWBP contained glycosidic linkages of T-<i>β</i>-D-Glc<i>f</i>, 1→2-<i>α</i>-D-Man<i>p</i>, 1→5,6-<i>β</i>-D-Gal<i>f</i>, 1→2,3,4-<i>α</i>-D-Gal<i>p</i> and 1→2,3,4-<i>β</i>-D-Man<i>p</i>, with triple-helix conformations. The morphological observation showed that IC-FWBP was composed of rod-like and spherical particles. These investigations on the structural properties of IC-FWBP will be beneficial to further research on the functional properties of wheat bran polysaccharides. |
| first_indexed | 2024-03-11T06:34:22Z |
| format | Article |
| id | doaj.art-de6b3f9a4cf74ebd93b1fb1e01963352 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T06:34:22Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-de6b3f9a4cf74ebd93b1fb1e019633522023-11-17T11:02:53ZengMDPI AGFermentation2311-56372023-03-019330910.3390/fermentation9030309Structural Properties of a Heteropolysaccharide Released from <i>Isaria cicadae Miq.</i> Solid-State Fermented Wheat BranRui Liu0Ruixin Liu1Xuebing Yan2Ningjie Li3Ming Li4Zijian Zhi5Boli Guo6Min Zhang7State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaInstitute of Food Science and Technology CAAS, Beijing 100193, ChinaFood Structure and Function (FSF) Research Group, Department of Food Technology, Safety and Health, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Gent, BelgiumInstitute of Food Science and Technology CAAS, Beijing 100193, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaThe work aimed to improve the extraction efficiency of wheat bran polysaccharide by solid-state fermentation using the bioactive fungus <i>Isaria cicadae Miq</i>. and identify the structural properties of fermented wheat bran polysaccharide (IC-FWBP). The polysaccharide fraction of IC-FWBP was isolated with an extraction yield of 2.88% and an average molecular weight of 3.31 × 10<sup>6</sup> Da. The IC-FWBP was comprised of mannose, glucose, and galactose. The methylation, nuclear magnetic resonance (NMR), and Congo red analysis results suggested that IC-FWBP contained glycosidic linkages of T-<i>β</i>-D-Glc<i>f</i>, 1→2-<i>α</i>-D-Man<i>p</i>, 1→5,6-<i>β</i>-D-Gal<i>f</i>, 1→2,3,4-<i>α</i>-D-Gal<i>p</i> and 1→2,3,4-<i>β</i>-D-Man<i>p</i>, with triple-helix conformations. The morphological observation showed that IC-FWBP was composed of rod-like and spherical particles. These investigations on the structural properties of IC-FWBP will be beneficial to further research on the functional properties of wheat bran polysaccharides.https://www.mdpi.com/2311-5637/9/3/309solid-state fermentationwheat branCordyceps fungipolysaccharidesstructural properties |
| spellingShingle | Rui Liu Ruixin Liu Xuebing Yan Ningjie Li Ming Li Zijian Zhi Boli Guo Min Zhang Structural Properties of a Heteropolysaccharide Released from <i>Isaria cicadae Miq.</i> Solid-State Fermented Wheat Bran Fermentation solid-state fermentation wheat bran Cordyceps fungi polysaccharides structural properties |
| title | Structural Properties of a Heteropolysaccharide Released from <i>Isaria cicadae Miq.</i> Solid-State Fermented Wheat Bran |
| title_full | Structural Properties of a Heteropolysaccharide Released from <i>Isaria cicadae Miq.</i> Solid-State Fermented Wheat Bran |
| title_fullStr | Structural Properties of a Heteropolysaccharide Released from <i>Isaria cicadae Miq.</i> Solid-State Fermented Wheat Bran |
| title_full_unstemmed | Structural Properties of a Heteropolysaccharide Released from <i>Isaria cicadae Miq.</i> Solid-State Fermented Wheat Bran |
| title_short | Structural Properties of a Heteropolysaccharide Released from <i>Isaria cicadae Miq.</i> Solid-State Fermented Wheat Bran |
| title_sort | structural properties of a heteropolysaccharide released from i isaria cicadae miq i solid state fermented wheat bran |
| topic | solid-state fermentation wheat bran Cordyceps fungi polysaccharides structural properties |
| url | https://www.mdpi.com/2311-5637/9/3/309 |
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