Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein

Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein

Summary: Successful genome editing in primary human islets could reveal features of the genetic regulatory landscape underlying β cell function and diabetes risk. Here, we describe a CRISPR-based strategy to interrogate functions of predicted regulatory DNA elements using electroporation of a comple...

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Bibliographic Details
Main Authors: Romina J. Bevacqua, Weichen Zhao, Emilio Merheb, Seung Hyun Kim, Alexander Marson, Anna L. Gloyn, Seung K. Kim
Format: Article
Language:English
Published: Elsevier 2024-01-01
Series:iScience
Subjects:
Techniques in genetics
human genetics
cell biology
biology experimental methods
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004223027700

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http://www.sciencedirect.com/science/article/pii/S2589004223027700

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