Successful CRISPR/Cas9-mediated HDR at individual DNA breakpoints using TFO-based targeted template design

Successful CRISPR/Cas9-mediated HDR at individual DNA breakpoints using TFO-based targeted template design

Background: Targeted insertion of the repair template into the genome is a common strategy for high-precision base replacements; however, the main challenge likely remains regarding the limited efficiency of homologous-directed repair (HDR). A precise genome cut achieved by CRISPR-Cas9 system combin...

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Bibliographic Details
Main Authors: Zahra Ebrahimi, Bahram Kazemi, Mohammad Salehi, Vahid Jajarmi
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:Electronic Journal of Biotechnology
Subjects:
CRISPR-Cas9
Donor template
Homologous-Directed Repair (HDR)
PolyPurine Reverse Hoogsteen hairpins (PPRHs)
Repair template
Repairing strategy
Online Access:http://www.sciencedirect.com/science/article/pii/S0717345824000022

Internet

http://www.sciencedirect.com/science/article/pii/S0717345824000022

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