Substituting Allose as the Primary Carbon Source During Enrichment Helps Improve Detection and Isolation of Lineage II Listeria monocytogenes From Food
Testing of foods for low levels of the human pathogen, Listeria monocytogenes (Lm), involves a selective enrichment procedure. A nonpathogenic species of Listeria, L. innocua (Li), is often present in foods and food-manufacturing environments and is an interference organism for Lm detection due to c...
| প্রধান লেখক: | , , , |
|---|---|
| বিন্যাস: | প্রবন্ধ |
| ভাষা: | English |
| প্রকাশিত: |
Elsevier
2023-07-01
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| মালা: | Journal of Food Protection |
| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | http://www.sciencedirect.com/science/article/pii/S0362028X23067765 |
| _version_ | 1827908295952695296 |
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| author | Jacqueline P. Upham Mikaela Eisebraun Alex Fortuna Gustavo V. Mallo |
| author_facet | Jacqueline P. Upham Mikaela Eisebraun Alex Fortuna Gustavo V. Mallo |
| author_sort | Jacqueline P. Upham |
| collection | DOAJ |
| description | Testing of foods for low levels of the human pathogen, Listeria monocytogenes (Lm), involves a selective enrichment procedure. A nonpathogenic species of Listeria, L. innocua (Li), is often present in foods and food-manufacturing environments and is an interference organism for Lm detection due to competition during enrichment. The present study investigated whether a novel enrichment strategy incorporating the sugar allose into the secondary enrichment broth (allose method) could improve the detection of Lm from foods when Li is present. First, Canadian food isolates of Listeria spp. were tested to confirm recent reports that lineage II Lm (LII-Lm), but not Li, could metabolize allose. All LII-Lm isolates (n = 81), but not Li (n = 36), possessed the allose genes lmo0734-lmo0739, and could efficiently metabolize allose. Next, smoked salmon was contaminated with mixtures of LII-Lm and Li and tested using different enrichment procedures to compare the ability to recover Lm. Allose broth was more effective than Fraser Broth, with Lm detected in 87% (74 of 85) compared to 59% (50 of 85) of the samples (P < 0.05), following a common preenrichment. When evaluated against a current Health Canada method (MFLP-28), the allose method was more effective, with LII-Lm detected in 88% (57 of 65) compared to 69% (45 of 65) of the samples (P < 0.05). The allose method also remarkably increased the ratio of LII-Lm to Li postenrichment, which improved the ease of obtaining isolated Lm colonies for confirmation tests. Allose may therefore provide a tool for use when the presence of background flora interferes with Lm detection. As this tool is specifically applicable to a subset of Lm, the use of this method modification may provide a working example of tailoring methodology to target the known subtype of the pathogen of interest in an outbreak investigation, or for regular monitoring activities in conjunction with a PCR screen for allose genes on preenrichment cultures. |
| first_indexed | 2024-03-13T01:19:53Z |
| format | Article |
| id | doaj.art-deb8c83e77884995b51a280df85a615d |
| institution | Directory Open Access Journal |
| issn | 0362-028X |
| language | English |
| last_indexed | 2024-03-13T01:19:53Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Food Protection |
| spelling | doaj.art-deb8c83e77884995b51a280df85a615d2023-07-05T05:13:59ZengElsevierJournal of Food Protection0362-028X2023-07-01867100104Substituting Allose as the Primary Carbon Source During Enrichment Helps Improve Detection and Isolation of Lineage II Listeria monocytogenes From FoodJacqueline P. Upham0Mikaela Eisebraun1Alex Fortuna2Gustavo V. Mallo3Canadian Food Inspection Agency - Dartmouth Laboratory, Dartmouth, Nova Scotia, Canada B3B 1Y9; Corresponding author.Canadian Food Inspection Agency - Dartmouth Laboratory, Dartmouth, Nova Scotia, Canada B3B 1Y9Ontario Institute for Cancer Research, Toronto, Ontario, Canada M5G 0A3Pathogen Preparedness and Test Development Unit, Public Health Ontario Laboratories, Toronto, Ontario, Canada M5G 1M1Testing of foods for low levels of the human pathogen, Listeria monocytogenes (Lm), involves a selective enrichment procedure. A nonpathogenic species of Listeria, L. innocua (Li), is often present in foods and food-manufacturing environments and is an interference organism for Lm detection due to competition during enrichment. The present study investigated whether a novel enrichment strategy incorporating the sugar allose into the secondary enrichment broth (allose method) could improve the detection of Lm from foods when Li is present. First, Canadian food isolates of Listeria spp. were tested to confirm recent reports that lineage II Lm (LII-Lm), but not Li, could metabolize allose. All LII-Lm isolates (n = 81), but not Li (n = 36), possessed the allose genes lmo0734-lmo0739, and could efficiently metabolize allose. Next, smoked salmon was contaminated with mixtures of LII-Lm and Li and tested using different enrichment procedures to compare the ability to recover Lm. Allose broth was more effective than Fraser Broth, with Lm detected in 87% (74 of 85) compared to 59% (50 of 85) of the samples (P < 0.05), following a common preenrichment. When evaluated against a current Health Canada method (MFLP-28), the allose method was more effective, with LII-Lm detected in 88% (57 of 65) compared to 69% (45 of 65) of the samples (P < 0.05). The allose method also remarkably increased the ratio of LII-Lm to Li postenrichment, which improved the ease of obtaining isolated Lm colonies for confirmation tests. Allose may therefore provide a tool for use when the presence of background flora interferes with Lm detection. As this tool is specifically applicable to a subset of Lm, the use of this method modification may provide a working example of tailoring methodology to target the known subtype of the pathogen of interest in an outbreak investigation, or for regular monitoring activities in conjunction with a PCR screen for allose genes on preenrichment cultures.http://www.sciencedirect.com/science/article/pii/S0362028X23067765AlloseLineage IIListeria monocytogeneslmo0734-lmo0739Smoked salmon |
| spellingShingle | Jacqueline P. Upham Mikaela Eisebraun Alex Fortuna Gustavo V. Mallo Substituting Allose as the Primary Carbon Source During Enrichment Helps Improve Detection and Isolation of Lineage II Listeria monocytogenes From Food Journal of Food Protection Allose Lineage II Listeria monocytogenes lmo0734-lmo0739 Smoked salmon |
| title | Substituting Allose as the Primary Carbon Source During Enrichment Helps Improve Detection and Isolation of Lineage II Listeria monocytogenes From Food |
| title_full | Substituting Allose as the Primary Carbon Source During Enrichment Helps Improve Detection and Isolation of Lineage II Listeria monocytogenes From Food |
| title_fullStr | Substituting Allose as the Primary Carbon Source During Enrichment Helps Improve Detection and Isolation of Lineage II Listeria monocytogenes From Food |
| title_full_unstemmed | Substituting Allose as the Primary Carbon Source During Enrichment Helps Improve Detection and Isolation of Lineage II Listeria monocytogenes From Food |
| title_short | Substituting Allose as the Primary Carbon Source During Enrichment Helps Improve Detection and Isolation of Lineage II Listeria monocytogenes From Food |
| title_sort | substituting allose as the primary carbon source during enrichment helps improve detection and isolation of lineage ii listeria monocytogenes from food |
| topic | Allose Lineage II Listeria monocytogenes lmo0734-lmo0739 Smoked salmon |
| url | http://www.sciencedirect.com/science/article/pii/S0362028X23067765 |
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