Comparison of different protocols for the bovine viral diarrhea virus detection by RT-PCR in pools of whole blood and blood serum artificially contaminated/ <br> Comparação de diferentes protocolos para a detecção do vírus da diarréia viral bovina por RT-PCR em grupos de sangue total e de soro sangüíneo, artificialmente contaminados
The RT-PCR technique was optimized and evaluated to detect the 5’ untranslated region of bovine viral diarrhea virus (BVDV) from clinical samples that consisted of blood serum and whole blood artificially contaminated with the NADL strain of BVDV. To optimization of technique, the following conditio...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Universidade Estadual de Londrina
2005-06-01
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Series: | Semina: Ciências Agrárias |
Subjects: | |
Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2295 |