Comparison of different protocols for the bovine viral diarrhea virus detection by RT-PCR in pools of whole blood and blood serum artificially contaminated/ <br> Comparação de diferentes protocolos para a detecção do vírus da diarréia viral bovina por RT-PCR em grupos de sangue total e de soro sangüíneo, artificialmente contaminados

Comparison of different protocols for the bovine viral diarrhea virus detection by RT-PCR in pools of whole blood and blood serum artificially contaminated/ <br> Comparação de diferentes protocolos para a detecção do vírus da diarréia viral bovina por RT-PCR em grupos de sangue total e de soro sangüíneo, artificialmente contaminados

The RT-PCR technique was optimized and evaluated to detect the 5’ untranslated region of bovine viral diarrhea virus (BVDV) from clinical samples that consisted of blood serum and whole blood artificially contaminated with the NADL strain of BVDV. To optimization of technique, the following conditio...

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Bibliographic Details
Main Authors: Amauri A. Alfieri, Alice F. Alfieri, Daniela Pilz
Format: Article
Language:English
Published: Universidade Estadual de Londrina 2005-06-01
Series:Semina: Ciências Agrárias
Subjects:
Bovino
Diarréia viral bovina
BVDV
RT-PCR
Soro sangüíneo.
Online Access:http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/2295

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