Cathepsin B Is Upregulated and Mediates ECM Degradation in Colon Adenocarcinoma HT29 Cells Overexpressing Snail
During tumor development and ongoing metastasis the acquisition of mesenchymal cell traits by epithelial carcinoma cells is achieved through a programmed phenotypic shift called the epithelial-to-mesenchymal transition, EMT. EMT contributes to increased cancer cell motility and invasiveness mainly t...
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| Format: | Article |
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MDPI AG
2019-02-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/3/203 |
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| author | Jakub Kryczka Izabela Papiewska-Pajak M. Anna Kowalska Joanna Boncela |
| author_facet | Jakub Kryczka Izabela Papiewska-Pajak M. Anna Kowalska Joanna Boncela |
| author_sort | Jakub Kryczka |
| collection | DOAJ |
| description | During tumor development and ongoing metastasis the acquisition of mesenchymal cell traits by epithelial carcinoma cells is achieved through a programmed phenotypic shift called the epithelial-to-mesenchymal transition, EMT. EMT contributes to increased cancer cell motility and invasiveness mainly through invadosomes, the adhesion structures that accompany the mesenchymal migration. The invadosomes and their associated proteases restrict protease activity to areas of the cell in direct contact with the ECM, thus precisely controlling cell invasion. Our data prove that Snail-overexpressing HT-29 cells that imitate the phenotype of colon cancer cells in the early stage of the EMT showed an increase in the expression and pericellular activity of cathepsin B. It appears that the pericellular localization of cathepsin B, also observed in colon and rectum adenocarcinoma tissue samples, plays a key role in its function. |
| first_indexed | 2024-03-12T19:45:39Z |
| format | Article |
| id | doaj.art-deca823bbb24496885e9764fa533c73b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T19:45:39Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-deca823bbb24496885e9764fa533c73b2023-08-02T03:34:20ZengMDPI AGCells2073-44092019-02-018320310.3390/cells8030203cells8030203Cathepsin B Is Upregulated and Mediates ECM Degradation in Colon Adenocarcinoma HT29 Cells Overexpressing SnailJakub Kryczka0Izabela Papiewska-Pajak1M. Anna Kowalska2Joanna Boncela3Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, PolandInstitute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, PolandInstitute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, PolandInstitute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, PolandDuring tumor development and ongoing metastasis the acquisition of mesenchymal cell traits by epithelial carcinoma cells is achieved through a programmed phenotypic shift called the epithelial-to-mesenchymal transition, EMT. EMT contributes to increased cancer cell motility and invasiveness mainly through invadosomes, the adhesion structures that accompany the mesenchymal migration. The invadosomes and their associated proteases restrict protease activity to areas of the cell in direct contact with the ECM, thus precisely controlling cell invasion. Our data prove that Snail-overexpressing HT-29 cells that imitate the phenotype of colon cancer cells in the early stage of the EMT showed an increase in the expression and pericellular activity of cathepsin B. It appears that the pericellular localization of cathepsin B, also observed in colon and rectum adenocarcinoma tissue samples, plays a key role in its function.https://www.mdpi.com/2073-4409/8/3/203EMTSnailmesenchymal type of migrationproteases |
| spellingShingle | Jakub Kryczka Izabela Papiewska-Pajak M. Anna Kowalska Joanna Boncela Cathepsin B Is Upregulated and Mediates ECM Degradation in Colon Adenocarcinoma HT29 Cells Overexpressing Snail Cells EMT Snail mesenchymal type of migration proteases |
| title | Cathepsin B Is Upregulated and Mediates ECM Degradation in Colon Adenocarcinoma HT29 Cells Overexpressing Snail |
| title_full | Cathepsin B Is Upregulated and Mediates ECM Degradation in Colon Adenocarcinoma HT29 Cells Overexpressing Snail |
| title_fullStr | Cathepsin B Is Upregulated and Mediates ECM Degradation in Colon Adenocarcinoma HT29 Cells Overexpressing Snail |
| title_full_unstemmed | Cathepsin B Is Upregulated and Mediates ECM Degradation in Colon Adenocarcinoma HT29 Cells Overexpressing Snail |
| title_short | Cathepsin B Is Upregulated and Mediates ECM Degradation in Colon Adenocarcinoma HT29 Cells Overexpressing Snail |
| title_sort | cathepsin b is upregulated and mediates ecm degradation in colon adenocarcinoma ht29 cells overexpressing snail |
| topic | EMT Snail mesenchymal type of migration proteases |
| url | https://www.mdpi.com/2073-4409/8/3/203 |
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