Construction of acetyl-CoA and DBAT hybrid metabolic pathway for acetylation of 10-deacetylbaccatin III to baccatin III

Construction of acetyl-CoA and DBAT hybrid metabolic pathway for acetylation of 10-deacetylbaccatin III to baccatin III

10-Deacetylbaccatin III (10-DAB) C10 acetylation is an indispensable procedure for Taxol semi-synthesis, which often requires harsh conditions. 10-Deacetylbaccatin III-10-β-O-acetyltransferase (DBAT) catalyzes the acetylation but acetyl-CoA supply remains a key limiting factor. Here we refactored th...

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Bibliographic Details
Main Authors: Hao Wang, Bo-Yong Zhang, Ting Gong, Tian-Jiao Chen, Jing-Jing Chen, Jin-Ling Yang, Ping Zhu
Format: Article
Language:English
Published: Elsevier 2021-10-01
Series:Acta Pharmaceutica Sinica B
Subjects:
Microbial cell factory
Biosynthetic pathway of acetyl-CoA
Bioconversion of 10-deacetylbaccatin III to baccatin III
10-Deacetylbaccatin III-10-β-O-acetyltransferase
Deacetylation of baccatin III
High-cell-density fermentation
Online Access:http://www.sciencedirect.com/science/article/pii/S2211383521001015

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