Spastin interacts with collapsin response mediator protein 3 to regulate neurite growth and branching
Cytoskeletal microtubule rearrangement and movement are crucial in the repair of spinal cord injury. Spastin plays an important role in the regulation of microtubule severing. Both spastin and collapsin response mediator proteins can regulate neurite growth and branching; however, whether spastin in...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2021;volume=16;issue=12;spage=2549;epage=2556;aulast=Ji |
| _version_ | 1829487853709557760 |
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| author | Zhi-Sheng Ji Jian-Ping Li Chao-Hua Fu Jian-Xian Luo Hua Yang Guo-Wei Zhang Wutian Wu Hong-Sheng Lin |
| author_facet | Zhi-Sheng Ji Jian-Ping Li Chao-Hua Fu Jian-Xian Luo Hua Yang Guo-Wei Zhang Wutian Wu Hong-Sheng Lin |
| author_sort | Zhi-Sheng Ji |
| collection | DOAJ |
| description | Cytoskeletal microtubule rearrangement and movement are crucial in the repair of spinal cord injury. Spastin plays an important role in the regulation of microtubule severing. Both spastin and collapsin response mediator proteins can regulate neurite growth and branching; however, whether spastin interacts with collapsin response mediator protein 3 (CRMP3) during this process remains unclear, as is the mechanism by which CRMP3 participates in the repair of spinal cord injury. In this study, we used a proteomics approach to identify key proteins associated with spinal cord injury repair. We then employed liquid chromatography-mass spectrometry to identify proteins that were able to interact with glutathione S-transferase-spastin. Then, co-immunoprecipitation and staining approaches were used to evaluate potential interactions between spastin and CRMP3. Finally, we co-transfected primary hippocampal neurons with CRMP3 and spastin to evaluate their role in neurite outgrowth. Mass spectrometry identified the role of CRMP3 in the spinal cord injury repair process. Liquid chromatography-mass spectrometry pulldown assays identified three CRMP3 peptides that were able to interact with spastin. CRMP3 and spastin were co-expressed in the spinal cord and were able to interact with one another in vitro and in vivo. Lastly, CRMP3 overexpression was able to enhance the ability of spastin to promote neurite growth and branching. Therefore, our results confirm that spastin and CRMP3 play roles in spinal cord injury repair by regulating neurite growth and branching. These proteins may therefore be novel targets for spinal cord injury repair. The Institutional Animal Care and Use Committee of Jinan University, China approved this study (approval No. IACUS-20181008-03) on October 8, 2018. |
| first_indexed | 2024-12-14T23:38:22Z |
| format | Article |
| id | doaj.art-def7d1e6d8bd4b51a1b92cd111c0b4bb |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-14T23:38:22Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-def7d1e6d8bd4b51a1b92cd111c0b4bb2022-12-21T22:43:35ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742021-01-0116122549255610.4103/1673-5374.313052Spastin interacts with collapsin response mediator protein 3 to regulate neurite growth and branchingZhi-Sheng JiJian-Ping LiChao-Hua FuJian-Xian LuoHua YangGuo-Wei ZhangWutian WuHong-Sheng LinCytoskeletal microtubule rearrangement and movement are crucial in the repair of spinal cord injury. Spastin plays an important role in the regulation of microtubule severing. Both spastin and collapsin response mediator proteins can regulate neurite growth and branching; however, whether spastin interacts with collapsin response mediator protein 3 (CRMP3) during this process remains unclear, as is the mechanism by which CRMP3 participates in the repair of spinal cord injury. In this study, we used a proteomics approach to identify key proteins associated with spinal cord injury repair. We then employed liquid chromatography-mass spectrometry to identify proteins that were able to interact with glutathione S-transferase-spastin. Then, co-immunoprecipitation and staining approaches were used to evaluate potential interactions between spastin and CRMP3. Finally, we co-transfected primary hippocampal neurons with CRMP3 and spastin to evaluate their role in neurite outgrowth. Mass spectrometry identified the role of CRMP3 in the spinal cord injury repair process. Liquid chromatography-mass spectrometry pulldown assays identified three CRMP3 peptides that were able to interact with spastin. CRMP3 and spastin were co-expressed in the spinal cord and were able to interact with one another in vitro and in vivo. Lastly, CRMP3 overexpression was able to enhance the ability of spastin to promote neurite growth and branching. Therefore, our results confirm that spastin and CRMP3 play roles in spinal cord injury repair by regulating neurite growth and branching. These proteins may therefore be novel targets for spinal cord injury repair. The Institutional Animal Care and Use Committee of Jinan University, China approved this study (approval No. IACUS-20181008-03) on October 8, 2018.http://www.nrronline.org/article.asp?issn=1673-5374;year=2021;volume=16;issue=12;spage=2549;epage=2556;aulast=Jicollapsin response mediator protein 3; liquid chromatography-mass spectrometry; microtubule; neurite growth; protein interactions; proteomics; spastin; spinal cord injury |
| spellingShingle | Zhi-Sheng Ji Jian-Ping Li Chao-Hua Fu Jian-Xian Luo Hua Yang Guo-Wei Zhang Wutian Wu Hong-Sheng Lin Spastin interacts with collapsin response mediator protein 3 to regulate neurite growth and branching Neural Regeneration Research collapsin response mediator protein 3; liquid chromatography-mass spectrometry; microtubule; neurite growth; protein interactions; proteomics; spastin; spinal cord injury |
| title | Spastin interacts with collapsin response mediator protein 3 to regulate neurite growth and branching |
| title_full | Spastin interacts with collapsin response mediator protein 3 to regulate neurite growth and branching |
| title_fullStr | Spastin interacts with collapsin response mediator protein 3 to regulate neurite growth and branching |
| title_full_unstemmed | Spastin interacts with collapsin response mediator protein 3 to regulate neurite growth and branching |
| title_short | Spastin interacts with collapsin response mediator protein 3 to regulate neurite growth and branching |
| title_sort | spastin interacts with collapsin response mediator protein 3 to regulate neurite growth and branching |
| topic | collapsin response mediator protein 3; liquid chromatography-mass spectrometry; microtubule; neurite growth; protein interactions; proteomics; spastin; spinal cord injury |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2021;volume=16;issue=12;spage=2549;epage=2556;aulast=Ji |
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