Structural and functional analysis of hyper-thermostable ancestral L-amino acid oxidase that can convert Trp derivatives to D-forms by chemoenzymatic reaction

Structural and functional analysis of hyper-thermostable ancestral L-amino acid oxidase that can convert Trp derivatives to D-forms by chemoenzymatic reaction

Abstract Production of D-amino acids (D-AAs) on a large-scale enables to provide precursors of peptide therapeutics. In this study, we designed a novel L-amino acid oxidase, HTAncLAAO2, by ancestral sequence reconstruction, exhibiting high thermostability and long-term stability. The crystal structu...

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Bibliographic Details
Main Authors: Yui Kawamura, Chiharu Ishida, Ryo Miyata, Azusa Miyata, Seiichiro Hayashi, Daisuke Fujinami, Sohei Ito, Shogo Nakano
Format: Article
Language:English
Published: Nature Portfolio 2023-09-01
Series:Communications Chemistry
Online Access:https://doi.org/10.1038/s42004-023-01005-1

Internet

https://doi.org/10.1038/s42004-023-01005-1

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