Validation of a Novel Double Control Quantitative Copy Number PCR Method to Quantify Off-Target Transgene Integration after CRISPR-Induced DNA Modification

Validation of a Novel Double Control Quantitative Copy Number PCR Method to Quantify Off-Target Transgene Integration after CRISPR-Induced DNA Modification

In order to improve a recently established cell-based assay to assess the potency of botulinum neurotoxin, neuroblastoma-derived SiMa cells and induced pluripotent stem-cells (iPSC) were modified to incorporate the coding sequence of a reporter luciferase into a genetic safe harbor utilizing CRISPR/...

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Bibliographic Details
Main Authors: Brit-Maren Michaud Schjeide, Maren Schenke, Bettina Seeger, Gerhard Paul Püschel
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Methods and Protocols
Subjects:
CRISPR editing validation
copy number analyses
homology-directed repair
homologous recombination deficiency
Online Access:https://www.mdpi.com/2409-9279/5/3/43

Internet

https://www.mdpi.com/2409-9279/5/3/43

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