Selective estrogen receptor modulators decrease invasiveness in pituitary adenoma cell lines AtT‐20 and TtT/GF by affecting expression of MMP‐14 and ADAM12
Selective estrogen receptor modulators (SERMs) significantly affect survival and invasiveness of rodent pituitary adenoma (PA) cells. The impact of three clinically relevant SERMs (bazedoxifene, clomiphene, raloxifene) on invasiveness and on gene and protein expression of invasion‐related proteases...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-11-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12999 |
| _version_ | 1818530275913629696 |
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| author | Zhuo Zhang Jörg W. Bartsch Julia Benzel Ting Lei Christopher Nimsky Benjamin Voellger |
| author_facet | Zhuo Zhang Jörg W. Bartsch Julia Benzel Ting Lei Christopher Nimsky Benjamin Voellger |
| author_sort | Zhuo Zhang |
| collection | DOAJ |
| description | Selective estrogen receptor modulators (SERMs) significantly affect survival and invasiveness of rodent pituitary adenoma (PA) cells. The impact of three clinically relevant SERMs (bazedoxifene, clomiphene, raloxifene) on invasiveness and on gene and protein expression of invasion‐related proteases [matrix metalloproteinase‐14 (MMP‐14) and A disintegrin and metalloproteinase‐12 (ADAM12)] was analyzed in murine PA cells (AtT‐20 and TtT/GF). All SERMs significantly decreased cell invasiveness. Moreover, SERMs significantly decreased expression of ADAM12 mRNA in both cell lines and of MMP‐14 mRNA in TtT/GF cells. Invasion rates of AtT‐20 and TtT/GF significantly decreased after ADAM12 gene silencing, and the invasion rate of TtT/GF cells significantly decreased after MMP‐14 gene silencing. All SERMs affected ADAM12 protein expression in AtT‐20 cells whereas bazedoxifene and raloxifene decreased MMP‐14 protein expression in TtT/GF cells. We conclude that SERMs attenuate invasiveness of murine PA cells by downregulating expression levels of invasion‐related proteases MMP‐14 and ADAM12. |
| first_indexed | 2024-12-11T17:17:40Z |
| format | Article |
| id | doaj.art-df26948269dd4c129cd7ad61e1cb41b9 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-12-11T17:17:40Z |
| publishDate | 2020-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-df26948269dd4c129cd7ad61e1cb41b92022-12-22T00:57:16ZengWileyFEBS Open Bio2211-54632020-11-0110112489249810.1002/2211-5463.12999Selective estrogen receptor modulators decrease invasiveness in pituitary adenoma cell lines AtT‐20 and TtT/GF by affecting expression of MMP‐14 and ADAM12Zhuo Zhang0Jörg W. Bartsch1Julia Benzel2Ting Lei3Christopher Nimsky4Benjamin Voellger5Department of Neurosurgery University Hospital Marburg GermanyDepartment of Neurosurgery University Hospital Marburg GermanyDepartment of Neurosurgery University Hospital Marburg GermanyDepartment of Neurosurgery Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology Wuhan ChinaDepartment of Neurosurgery University Hospital Marburg GermanyDepartment of Neurosurgery University Hospital Marburg GermanySelective estrogen receptor modulators (SERMs) significantly affect survival and invasiveness of rodent pituitary adenoma (PA) cells. The impact of three clinically relevant SERMs (bazedoxifene, clomiphene, raloxifene) on invasiveness and on gene and protein expression of invasion‐related proteases [matrix metalloproteinase‐14 (MMP‐14) and A disintegrin and metalloproteinase‐12 (ADAM12)] was analyzed in murine PA cells (AtT‐20 and TtT/GF). All SERMs significantly decreased cell invasiveness. Moreover, SERMs significantly decreased expression of ADAM12 mRNA in both cell lines and of MMP‐14 mRNA in TtT/GF cells. Invasion rates of AtT‐20 and TtT/GF significantly decreased after ADAM12 gene silencing, and the invasion rate of TtT/GF cells significantly decreased after MMP‐14 gene silencing. All SERMs affected ADAM12 protein expression in AtT‐20 cells whereas bazedoxifene and raloxifene decreased MMP‐14 protein expression in TtT/GF cells. We conclude that SERMs attenuate invasiveness of murine PA cells by downregulating expression levels of invasion‐related proteases MMP‐14 and ADAM12.https://doi.org/10.1002/2211-5463.12999ADAM proteasesanti‐estrogensinvasivenessmatrix metalloproteinasespituitary adenoma |
| spellingShingle | Zhuo Zhang Jörg W. Bartsch Julia Benzel Ting Lei Christopher Nimsky Benjamin Voellger Selective estrogen receptor modulators decrease invasiveness in pituitary adenoma cell lines AtT‐20 and TtT/GF by affecting expression of MMP‐14 and ADAM12 FEBS Open Bio ADAM proteases anti‐estrogens invasiveness matrix metalloproteinases pituitary adenoma |
| title | Selective estrogen receptor modulators decrease invasiveness in pituitary adenoma cell lines AtT‐20 and TtT/GF by affecting expression of MMP‐14 and ADAM12 |
| title_full | Selective estrogen receptor modulators decrease invasiveness in pituitary adenoma cell lines AtT‐20 and TtT/GF by affecting expression of MMP‐14 and ADAM12 |
| title_fullStr | Selective estrogen receptor modulators decrease invasiveness in pituitary adenoma cell lines AtT‐20 and TtT/GF by affecting expression of MMP‐14 and ADAM12 |
| title_full_unstemmed | Selective estrogen receptor modulators decrease invasiveness in pituitary adenoma cell lines AtT‐20 and TtT/GF by affecting expression of MMP‐14 and ADAM12 |
| title_short | Selective estrogen receptor modulators decrease invasiveness in pituitary adenoma cell lines AtT‐20 and TtT/GF by affecting expression of MMP‐14 and ADAM12 |
| title_sort | selective estrogen receptor modulators decrease invasiveness in pituitary adenoma cell lines att 20 and ttt gf by affecting expression of mmp 14 and adam12 |
| topic | ADAM proteases anti‐estrogens invasiveness matrix metalloproteinases pituitary adenoma |
| url | https://doi.org/10.1002/2211-5463.12999 |
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