Comparative transcriptomic analysis of two Cucumis melo var. saccharinus germplasms differing in fruit physical and chemical characteristics
Abstract Background Hami melon (Cucumis melo var. saccharinus) is a popular fruit in China because of its excellent taste, which is largely determined by its physicochemical characteristics, including flesh texture, sugar content, aroma, and nutrient composition. However, the mechanisms by which the...
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-022-03550-8 |
| _version_ | 1818480695815700480 |
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| author | Renfan Liang Yicheng Su Xiaojuan Qin Zhongkui Gao Zhixin Fu Huijun Qiu Xu Lin Jinlian Zhu |
| author_facet | Renfan Liang Yicheng Su Xiaojuan Qin Zhongkui Gao Zhixin Fu Huijun Qiu Xu Lin Jinlian Zhu |
| author_sort | Renfan Liang |
| collection | DOAJ |
| description | Abstract Background Hami melon (Cucumis melo var. saccharinus) is a popular fruit in China because of its excellent taste, which is largely determined by its physicochemical characteristics, including flesh texture, sugar content, aroma, and nutrient composition. However, the mechanisms by which these characteristics are regulated have not yet been determined. In this study, we monitored changes in the fruits of two germplasms that differed in physicochemical characteristics throughout the fruit development period. Results Ripe fruit of the bred variety ‘Guimi’ had significantly higher soluble sugar contents than the fruit of the common variety ‘Yaolong.’ Additionally, differences in fruit shape and color between these two germplasms were observed during development. Comparative transcriptome analysis, conducted to identify regulators and pathways underlying the observed differences at corresponding stages of development, revealed a higher number of differentially expressed genes (DEGs) in Guimi than in Yaolong. Moreover, most DEGs detected during early fruit development in Guimi were associated with cell wall biogenesis. Temporal analysis of the identified DEGs revealed similar trends in the enrichment of downregulated genes in both germplasms, although there were differences in the enrichment trends of upregulated genes. Further analyses revealed trends in differential changes in multiple genes involved in cell wall biogenesis and sugar metabolism during fruit ripening. Conclusions We identified several genes associated with the ripening of Hami melons, which will provide novel insights into the molecular mechanisms underlying the development of fruit characteristics in these melons. |
| first_indexed | 2024-12-10T11:26:06Z |
| format | Article |
| id | doaj.art-df34ddf9bdd74d56aefe0b779479d698 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-10T11:26:06Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-df34ddf9bdd74d56aefe0b779479d6982022-12-22T01:50:45ZengBMCBMC Plant Biology1471-22292022-04-0122111210.1186/s12870-022-03550-8Comparative transcriptomic analysis of two Cucumis melo var. saccharinus germplasms differing in fruit physical and chemical characteristicsRenfan Liang0Yicheng Su1Xiaojuan Qin2Zhongkui Gao3Zhixin Fu4Huijun Qiu5Xu Lin6Jinlian Zhu7Guangxi Academy of Agricultural SciencesGuangxi Academy of Agricultural SciencesGuangxi Academy of Agricultural SciencesGuangxi Academy of Agricultural SciencesGuangxi Academy of Agricultural SciencesGuangxi Academy of Agricultural SciencesGuangxi Academy of Agricultural SciencesGuangxi Normal University for NationalitiesAbstract Background Hami melon (Cucumis melo var. saccharinus) is a popular fruit in China because of its excellent taste, which is largely determined by its physicochemical characteristics, including flesh texture, sugar content, aroma, and nutrient composition. However, the mechanisms by which these characteristics are regulated have not yet been determined. In this study, we monitored changes in the fruits of two germplasms that differed in physicochemical characteristics throughout the fruit development period. Results Ripe fruit of the bred variety ‘Guimi’ had significantly higher soluble sugar contents than the fruit of the common variety ‘Yaolong.’ Additionally, differences in fruit shape and color between these two germplasms were observed during development. Comparative transcriptome analysis, conducted to identify regulators and pathways underlying the observed differences at corresponding stages of development, revealed a higher number of differentially expressed genes (DEGs) in Guimi than in Yaolong. Moreover, most DEGs detected during early fruit development in Guimi were associated with cell wall biogenesis. Temporal analysis of the identified DEGs revealed similar trends in the enrichment of downregulated genes in both germplasms, although there were differences in the enrichment trends of upregulated genes. Further analyses revealed trends in differential changes in multiple genes involved in cell wall biogenesis and sugar metabolism during fruit ripening. Conclusions We identified several genes associated with the ripening of Hami melons, which will provide novel insights into the molecular mechanisms underlying the development of fruit characteristics in these melons.https://doi.org/10.1186/s12870-022-03550-8Cucumis melo varSaccharinusSoluble sugarPhysical characteristicsFruit ripeningTranscriptome |
| spellingShingle | Renfan Liang Yicheng Su Xiaojuan Qin Zhongkui Gao Zhixin Fu Huijun Qiu Xu Lin Jinlian Zhu Comparative transcriptomic analysis of two Cucumis melo var. saccharinus germplasms differing in fruit physical and chemical characteristics BMC Plant Biology Cucumis melo var Saccharinus Soluble sugar Physical characteristics Fruit ripening Transcriptome |
| title | Comparative transcriptomic analysis of two Cucumis melo var. saccharinus germplasms differing in fruit physical and chemical characteristics |
| title_full | Comparative transcriptomic analysis of two Cucumis melo var. saccharinus germplasms differing in fruit physical and chemical characteristics |
| title_fullStr | Comparative transcriptomic analysis of two Cucumis melo var. saccharinus germplasms differing in fruit physical and chemical characteristics |
| title_full_unstemmed | Comparative transcriptomic analysis of two Cucumis melo var. saccharinus germplasms differing in fruit physical and chemical characteristics |
| title_short | Comparative transcriptomic analysis of two Cucumis melo var. saccharinus germplasms differing in fruit physical and chemical characteristics |
| title_sort | comparative transcriptomic analysis of two cucumis melo var saccharinus germplasms differing in fruit physical and chemical characteristics |
| topic | Cucumis melo var Saccharinus Soluble sugar Physical characteristics Fruit ripening Transcriptome |
| url | https://doi.org/10.1186/s12870-022-03550-8 |
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