Using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset

Summary: The microtubule (MT)-stabilizing drug Taxol (paclitaxel) is a commonly used tool to investigate MT dynamics and MT-dependent processes. Here, we present a protocol for using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset. We describe...

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Bibliographic Details
Main Authors: Angela R. Bunning, Samuel J. Anderson, Mohan L. Gupta, Jr.
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723004896