Real-Time fast PCR amplification using designated and conventional real time thermal cycler systems: COVID-19 perspective.

Real-Time fast PCR amplification using designated and conventional real time thermal cycler systems: COVID-19 perspective.

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The study aimed to shorten multiplex RT-PCR run time for detection of SARS CoV-2 N1 and N2 sequences and human RNase P (RP) sequence as internal mRNA control using conventional and designated real time thermal cycler systems. Optimization of Fast PCR protocol using plasmid-based N1 and N2 positive c...

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Bibliographic Details
Main Authors: Md Walid Hossain, Mohabbat Hossain, Khalid Arafath, Subarna Sayed Ety, Md Mahade Hasan Shetu, Mazbahul Kabir, Farjana Akther Noor, Kaiissar Mannoor
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2022-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0276464

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https://doi.org/10.1371/journal.pone.0276464

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