Hematin and CuII control generations of hydroperoxyl and superoxide radicals to activate galactose oxidase for 5-hydroxymethylfurfural conversion
Summary: We present a significant finding that Cu(II) ions can activate hematin (Hem) to generate more HOO⋅ and O2⋅- radicals from the decomposition of H2O2. Galactose oxidase (GO) and hematin have been simultaneously immobilized by coordinating to Cu(II) ions (GO&Hem@Cu(II)). The radicals HOO⋅...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223025294 |
| _version_ | 1797388776099545088 |
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| author | Yao Chen Jinxin Zou Peijun Ji Wei Feng |
| author_facet | Yao Chen Jinxin Zou Peijun Ji Wei Feng |
| author_sort | Yao Chen |
| collection | DOAJ |
| description | Summary: We present a significant finding that Cu(II) ions can activate hematin (Hem) to generate more HOO⋅ and O2⋅- radicals from the decomposition of H2O2. Galactose oxidase (GO) and hematin have been simultaneously immobilized by coordinating to Cu(II) ions (GO&Hem@Cu(II)). The radicals HOO⋅ and O2⋅- and dioxygen O2 can be in situ generated from the byproduct H2O2 by the Cu(II)-activated hematin. Ample experimental evidence supports the discovery that the immobilized GO is reactivated by the in situ generated HOO⋅ and O2⋅-. For the conversion of 100 mM 5-hydroxymethylfurfural (HMF) in water, GO&Hem@Cu(II) (0.8 mg/mL GO encapsulated) has achieved a 99.5% conversion within 180 min. In contrast, 0.8 mg/mL free GO M3-5 variant (ACS Catalysis 2018, 8, 4025) has achieved an HMF conversion of 17.3%. For the conversion of HMF (1,000 mM) by GO&Hem@Cu(II) (4 mg/mL GO encapsulated), the HMF conversion is 98.8% after 8 h reaction. |
| first_indexed | 2024-03-08T22:45:43Z |
| format | Article |
| id | doaj.art-df9786b86f7b4314ac84d6c4eb3429fb |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T22:45:43Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-df9786b86f7b4314ac84d6c4eb3429fb2023-12-17T06:40:52ZengElsevieriScience2589-00422023-12-012612108452Hematin and CuII control generations of hydroperoxyl and superoxide radicals to activate galactose oxidase for 5-hydroxymethylfurfural conversionYao Chen0Jinxin Zou1Peijun Ji2Wei Feng3Department of Biological Engineering, Beijing University of Chemical Technology, Beijing, ChinaDepartment of Biological Engineering, Beijing University of Chemical Technology, Beijing, ChinaDepartment of Chemical Engineering, Beijing University of Chemical Technology, Beijing, ChinaDepartment of Biological Engineering, Beijing University of Chemical Technology, Beijing, China; Corresponding authorSummary: We present a significant finding that Cu(II) ions can activate hematin (Hem) to generate more HOO⋅ and O2⋅- radicals from the decomposition of H2O2. Galactose oxidase (GO) and hematin have been simultaneously immobilized by coordinating to Cu(II) ions (GO&Hem@Cu(II)). The radicals HOO⋅ and O2⋅- and dioxygen O2 can be in situ generated from the byproduct H2O2 by the Cu(II)-activated hematin. Ample experimental evidence supports the discovery that the immobilized GO is reactivated by the in situ generated HOO⋅ and O2⋅-. For the conversion of 100 mM 5-hydroxymethylfurfural (HMF) in water, GO&Hem@Cu(II) (0.8 mg/mL GO encapsulated) has achieved a 99.5% conversion within 180 min. In contrast, 0.8 mg/mL free GO M3-5 variant (ACS Catalysis 2018, 8, 4025) has achieved an HMF conversion of 17.3%. For the conversion of HMF (1,000 mM) by GO&Hem@Cu(II) (4 mg/mL GO encapsulated), the HMF conversion is 98.8% after 8 h reaction.http://www.sciencedirect.com/science/article/pii/S2589004223025294ChemistryChemical engineeringBiomass |
| spellingShingle | Yao Chen Jinxin Zou Peijun Ji Wei Feng Hematin and CuII control generations of hydroperoxyl and superoxide radicals to activate galactose oxidase for 5-hydroxymethylfurfural conversion iScience Chemistry Chemical engineering Biomass |
| title | Hematin and CuII control generations of hydroperoxyl and superoxide radicals to activate galactose oxidase for 5-hydroxymethylfurfural conversion |
| title_full | Hematin and CuII control generations of hydroperoxyl and superoxide radicals to activate galactose oxidase for 5-hydroxymethylfurfural conversion |
| title_fullStr | Hematin and CuII control generations of hydroperoxyl and superoxide radicals to activate galactose oxidase for 5-hydroxymethylfurfural conversion |
| title_full_unstemmed | Hematin and CuII control generations of hydroperoxyl and superoxide radicals to activate galactose oxidase for 5-hydroxymethylfurfural conversion |
| title_short | Hematin and CuII control generations of hydroperoxyl and superoxide radicals to activate galactose oxidase for 5-hydroxymethylfurfural conversion |
| title_sort | hematin and cuii control generations of hydroperoxyl and superoxide radicals to activate galactose oxidase for 5 hydroxymethylfurfural conversion |
| topic | Chemistry Chemical engineering Biomass |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223025294 |
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