Methodology: an optimized, high-yield tomato leaf chloroplast isolation and stroma extraction protocol for proteomics analyses and identification of chloroplast co-localizing proteins

Methodology: an optimized, high-yield tomato leaf chloroplast isolation and stroma extraction protocol for proteomics analyses and identification of chloroplast co-localizing proteins

Abstract Background Chloroplasts are critical organelles that perceive and convey metabolic and stress signals to different cellular components, while remaining the seat of photosynthesis and a metabolic factory. The proteomes of intact leaves, chloroplasts, and suborganellar fractions of plastids h...

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Bibliographic Details
Main Authors: Oindrila Bhattacharya, Irma Ortiz, Linda L. Walling
Format: Article
Language:English
Published: BMC 2020-09-01
Series:Plant Methods
Subjects:
Solanum lycopersicum
Chloroplast isolation
Stroma
Soluble proteins
Proteomics
Online Access:http://link.springer.com/article/10.1186/s13007-020-00667-5

Internet

http://link.springer.com/article/10.1186/s13007-020-00667-5

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