Three-dimensional biofilm colony growth supports a mutualism involving matrix and nutrient sharing

Three-dimensional biofilm colony growth supports a mutualism involving matrix and nutrient sharing

Life in a three-dimensional biofilm is typical for many bacteria, yet little is known about how strains interact in this context. Here, we created essential gene CRISPR interference knockdown libraries in biofilm-forming Bacillus subtilis and measured competitive fitness during colony co-culture wit...

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Bibliographic Details
Main Authors: Heidi A Arjes, Lisa Willis, Haiwen Gui, Yangbo Xiao, Jason Peters, Carol Gross, Kerwyn Casey Huang
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-02-01
Series:eLife
Subjects:
essential genes
CRISPRi
AlrA
D-alanine
extracellular matrix
surface growth
Online Access:https://elifesciences.org/articles/64145

Internet

https://elifesciences.org/articles/64145

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