Identification of Appropriate Endogenous Controls for Circulating miRNA Quantification in Working Dogs under Physiological Stress Conditions

Identification of Appropriate Endogenous Controls for Circulating miRNA Quantification in Working Dogs under Physiological Stress Conditions

Cell-free miRNAs, called circulating miRNAs (cmiRNAs), can act in a paracrine manner by facilitating a diversity of signaling mechanisms between cells. Real-time qPCR is the most accepted method for quantifying miRNA expression levels. The use of stable miRNA endogenous control (EC) for qPCR data no...

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Bibliographic Details
Main Authors: Gabriella Guelfi, Camilla Capaccia, Michele Matteo Santoro, Silvana Diverio
Format: Article
Language:English
Published: MDPI AG 2023-02-01
Series:Animals
Subjects:
endogenous control
qPCR
circulating miRNA
search and rescue dogs
physiological stress
Online Access:https://www.mdpi.com/2076-2615/13/4/576

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https://www.mdpi.com/2076-2615/13/4/576

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