Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

Abstract Background An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants. Although rice protoplasts can be isolated from germinated seedlings or cell suspension culture, p...

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Bibliographic Details
Main Authors: Snigdha Poddar, Jaclyn Tanaka, Jamie H. D. Cate, Brian Staskawicz, Myeong-Je Cho
Format: Article
Language:English
Published: BMC 2020-11-01
Series:Plant Methods
Subjects:
Protoplast isolation
Calli
Pause point
Transfection
Genome editing assay
Rice
Online Access:http://link.springer.com/article/10.1186/s13007-020-00692-4

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http://link.springer.com/article/10.1186/s13007-020-00692-4

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