A new approach to evaluate immobilization of β-galactosidase on Eupergit® C: structural, kinetic, and thermal characterization

This study aimed to evaluate β-galactosidase immobilization. For this purpose, the ionic strength of the buffer, reaction time, amount of the immobilization support, and pH were evaluated by a central composite design. Assay 8, which consisted of 1.5 mol L-1 phosphate buffer (pH 7.5) and a reaction...

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Bibliographic Details
Main Authors: Anna R. C. Braga, Marceli F. Silva, José V. Oliveira, Helen Treichel, Susana J. Kalil
Format: Article
Language:English
Published: Sociedade Brasileira de Química 2014-06-01
Series:Química Nova
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000500007&lng=en&tlng=en