Tracking and Quantitation of Retroviral-Mediated Transfer Using a Completely Humanized, Red-Shifted Green Fluorescent Protein Gene
We have developed murine retroviral vectors (RVs) containing an optimized green fluorescent protein (GFP) gene to study retroviral gene transfer and expression in living cells. We used the codon “humanized”, “red-shifted” GFP gene, hGFPS65T, a gain of function variant of the wild-type GFP from the j...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1997-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/97221rr03 |