Immunoprotection in sheep against Haemonchus contortus using its thiol-purified excretory/secretory proteins
Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chr...
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| Format: | Article |
| Language: | English |
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Urmia University
2012-12-01
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| Series: | Veterinary Research Forum |
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| Online Access: | http://www.urmia.ac.ir/vrf/Shared%20Documents/pdf/vol-3%20no-4/239-244-0145.pdf |