Disregarded determinant role of transfection medium in chitosan mediated siRNA delivery
It is well known that the chitosan (CS) properties such as molecular weight (Mw) and deacetylation degree (DD) are crucial for siRNA delivery. In the present study, we have unintentionally found that the transfection medium has much more substantial effect, which has not been reported before. Specif...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Materials & Design |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127522003707 |
| _version_ | 1811334597775982592 |
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| author | Wen Song Qingyuan Ye Zhe Li Yajie Hou Lina Niu Zhiwei Ma Chuanxu Yang |
| author_facet | Wen Song Qingyuan Ye Zhe Li Yajie Hou Lina Niu Zhiwei Ma Chuanxu Yang |
| author_sort | Wen Song |
| collection | DOAJ |
| description | It is well known that the chitosan (CS) properties such as molecular weight (Mw) and deacetylation degree (DD) are crucial for siRNA delivery. In the present study, we have unintentionally found that the transfection medium has much more substantial effect, which has not been reported before. Specifically, the transfection efficiency was considerably increased in Hank’s Balanced Saline Solution (HBSS), with various Mw (30–150 kD) and DD (85–95%) combinations in different cell types. In addition, even though the CS/siRNA nanoparticles physical parameters such as diameter size and polydisperse index were disturbed by cell culture medium or serum, a relatively high knockdown efficiency could still be obtained in HBSS. Moreover, the highly efficient silencing in HBSS even did not require pre-mixing to formulate the CS/siRNA nanoparticles. In conclusion, our findings have confirmed a neglected but crucial important factor of transfection medium in the chitosan mediated siRNA delivery. |
| first_indexed | 2024-04-13T17:11:17Z |
| format | Article |
| id | doaj.art-e0af7edeb2a943b78c56458c8ef30037 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-04-13T17:11:17Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-e0af7edeb2a943b78c56458c8ef300372022-12-22T02:38:17ZengElsevierMaterials & Design0264-12752022-07-01219110748Disregarded determinant role of transfection medium in chitosan mediated siRNA deliveryWen Song0Qingyuan Ye1Zhe Li2Yajie Hou3Lina Niu4Zhiwei Ma5Chuanxu Yang6State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China; Corresponding authors.State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China; Corresponding authors.Key Laboratory Colloid and Interface Chemistry of Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China; Corresponding authors.It is well known that the chitosan (CS) properties such as molecular weight (Mw) and deacetylation degree (DD) are crucial for siRNA delivery. In the present study, we have unintentionally found that the transfection medium has much more substantial effect, which has not been reported before. Specifically, the transfection efficiency was considerably increased in Hank’s Balanced Saline Solution (HBSS), with various Mw (30–150 kD) and DD (85–95%) combinations in different cell types. In addition, even though the CS/siRNA nanoparticles physical parameters such as diameter size and polydisperse index were disturbed by cell culture medium or serum, a relatively high knockdown efficiency could still be obtained in HBSS. Moreover, the highly efficient silencing in HBSS even did not require pre-mixing to formulate the CS/siRNA nanoparticles. In conclusion, our findings have confirmed a neglected but crucial important factor of transfection medium in the chitosan mediated siRNA delivery.http://www.sciencedirect.com/science/article/pii/S0264127522003707ChitosansiRNA deliveryMesenchymal stem cellsTransfection mediumHBSS |
| spellingShingle | Wen Song Qingyuan Ye Zhe Li Yajie Hou Lina Niu Zhiwei Ma Chuanxu Yang Disregarded determinant role of transfection medium in chitosan mediated siRNA delivery Materials & Design Chitosan siRNA delivery Mesenchymal stem cells Transfection medium HBSS |
| title | Disregarded determinant role of transfection medium in chitosan mediated siRNA delivery |
| title_full | Disregarded determinant role of transfection medium in chitosan mediated siRNA delivery |
| title_fullStr | Disregarded determinant role of transfection medium in chitosan mediated siRNA delivery |
| title_full_unstemmed | Disregarded determinant role of transfection medium in chitosan mediated siRNA delivery |
| title_short | Disregarded determinant role of transfection medium in chitosan mediated siRNA delivery |
| title_sort | disregarded determinant role of transfection medium in chitosan mediated sirna delivery |
| topic | Chitosan siRNA delivery Mesenchymal stem cells Transfection medium HBSS |
| url | http://www.sciencedirect.com/science/article/pii/S0264127522003707 |
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