Optimized protocol for stable isotope tracing and steady-state metabolomics in mouse HER2+ breast cancer brain metastasis

Optimized protocol for stable isotope tracing and steady-state metabolomics in mouse HER2+ breast cancer brain metastasis

Summary: Analyzing the metabolic dependencies of tumor cells is vital for cancer diagnosis and treatment. Here, we describe a protocol for 13C-stable glucose and glutamine isotope tracing in mice HER2+ breast cancer brain metastatic lesions. We describe how to inject cancer cells intracardially to g...

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Bibliographic Details
Main Authors: Pravat Kumar Parida, Mauricio Marquez-Palencia, Akash K. Kaushik, Kangsan Kim, Vidhya Nair, Jessica Sudderth, Hieu Vu, Lauren Zacharias, Ralph DeBerardinis, Srinivas Malladi
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cancer
Metabolism
Metabolomics
Mass Spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002258

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http://www.sciencedirect.com/science/article/pii/S2666166722002258

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