Detection of macrolide and fluoroquinolone resistance-associated 23S rRNA and parC mutations in Mycoplasma genitalium by nested real-time PCR

Detection of macrolide and fluoroquinolone resistance-associated 23S rRNA and parC mutations in Mycoplasma genitalium by nested real-time PCR

BackgroundTraditional drug susceptibility testing cannot be performed in clinical laboratories due to the slow-growing characteristics of Mycoplasma genitalium when cultured in vitro. Sanger sequencing is the standard method for detecting drug resistance-associated mutations. It has been used in som...

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Bibliographic Details
Main Authors: Wenyin He, Ying Yuan, Junyu Liang, Xuejiao Fan, Lei Li, Xingfei Pan
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-10-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
Mycoplasma genitalium
macrolide
fluroquinolone
23S rRNA
parC
nested real-time PCR
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2023.1271392/full

Internet

https://www.frontiersin.org/articles/10.3389/fcimb.2023.1271392/full

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