Puerarin Exerts Protective Effects on Wear Particle-Induced Inflammatory Osteolysis
Wear particle-stimulated inflammatory bone destruction and the consequent aseptic loosening remain major postoperative problems for artificial joints. Studies have indicated that puerarin promotes osteogenesis and alleviates lipopolysaccharide-induced osteoclastogenesis in vitro. However, the underl...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2019.01113/full |
| _version_ | 1818429825332805632 |
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| author | Chao Yang Juehong Li Kechao Zhu Xiangwei Yuan Tao Cheng Yebin Qian Xianlong Zhang |
| author_facet | Chao Yang Juehong Li Kechao Zhu Xiangwei Yuan Tao Cheng Yebin Qian Xianlong Zhang |
| author_sort | Chao Yang |
| collection | DOAJ |
| description | Wear particle-stimulated inflammatory bone destruction and the consequent aseptic loosening remain major postoperative problems for artificial joints. Studies have indicated that puerarin promotes osteogenesis and alleviates lipopolysaccharide-induced osteoclastogenesis in vitro. However, the underlying molecular mechanism by which puerarin interacts with receptor activator of nuclear factor kappa-B ligand (RANKL)-mediated osteoclast formation in vitro and wear particle-stimulated osteolysis in vivo has not been reported. In this work, the protective effects exerted by puerarin on titanium particle-stimulated bone destruction in vivo and on RANKL-induced osteoclast activation in osteoclastic precursor cells in vitro were investigated. As expected, puerarin significantly inhibited wear particle-mediated bone resorption and proinflammatory cytokine productions in a calvarial resorption model. Additionally, puerarin inhibited RANKL-induced osteoclast activation, bone resorption ability, and F-actin ring formation in vitro as puerarin concentration increased. Furthermore, mechanistic investigation indicated that reduced RANKL-stimulated MEK/ERK/NFATc1 signaling cascades might regulate the protective effect of puerarin. Conclusively, these results indicate that puerarin, a type of polyphenol, might serve as a protective agent to prevent osteoclast-related osteolytic diseases. |
| first_indexed | 2024-12-14T15:23:40Z |
| format | Article |
| id | doaj.art-e0f09bff7e6a4f7c953e06b6beefd181 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-14T15:23:40Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-e0f09bff7e6a4f7c953e06b6beefd1812022-12-21T22:56:05ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122019-10-011010.3389/fphar.2019.01113465918Puerarin Exerts Protective Effects on Wear Particle-Induced Inflammatory OsteolysisChao YangJuehong LiKechao ZhuXiangwei YuanTao ChengYebin QianXianlong ZhangWear particle-stimulated inflammatory bone destruction and the consequent aseptic loosening remain major postoperative problems for artificial joints. Studies have indicated that puerarin promotes osteogenesis and alleviates lipopolysaccharide-induced osteoclastogenesis in vitro. However, the underlying molecular mechanism by which puerarin interacts with receptor activator of nuclear factor kappa-B ligand (RANKL)-mediated osteoclast formation in vitro and wear particle-stimulated osteolysis in vivo has not been reported. In this work, the protective effects exerted by puerarin on titanium particle-stimulated bone destruction in vivo and on RANKL-induced osteoclast activation in osteoclastic precursor cells in vitro were investigated. As expected, puerarin significantly inhibited wear particle-mediated bone resorption and proinflammatory cytokine productions in a calvarial resorption model. Additionally, puerarin inhibited RANKL-induced osteoclast activation, bone resorption ability, and F-actin ring formation in vitro as puerarin concentration increased. Furthermore, mechanistic investigation indicated that reduced RANKL-stimulated MEK/ERK/NFATc1 signaling cascades might regulate the protective effect of puerarin. Conclusively, these results indicate that puerarin, a type of polyphenol, might serve as a protective agent to prevent osteoclast-related osteolytic diseases.https://www.frontiersin.org/article/10.3389/fphar.2019.01113/fulltitanium particlesosteolysispuerarinosteoclastogenesisERK pathway |
| spellingShingle | Chao Yang Juehong Li Kechao Zhu Xiangwei Yuan Tao Cheng Yebin Qian Xianlong Zhang Puerarin Exerts Protective Effects on Wear Particle-Induced Inflammatory Osteolysis Frontiers in Pharmacology titanium particles osteolysis puerarin osteoclastogenesis ERK pathway |
| title | Puerarin Exerts Protective Effects on Wear Particle-Induced Inflammatory Osteolysis |
| title_full | Puerarin Exerts Protective Effects on Wear Particle-Induced Inflammatory Osteolysis |
| title_fullStr | Puerarin Exerts Protective Effects on Wear Particle-Induced Inflammatory Osteolysis |
| title_full_unstemmed | Puerarin Exerts Protective Effects on Wear Particle-Induced Inflammatory Osteolysis |
| title_short | Puerarin Exerts Protective Effects on Wear Particle-Induced Inflammatory Osteolysis |
| title_sort | puerarin exerts protective effects on wear particle induced inflammatory osteolysis |
| topic | titanium particles osteolysis puerarin osteoclastogenesis ERK pathway |
| url | https://www.frontiersin.org/article/10.3389/fphar.2019.01113/full |
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