Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from <i>Ocotea indecora</i> (Shott) Mez in <i>Aspergillus</i> Species
The <i>Aspergillus</i> genus, the etiological agent of aspergillosis, is an important food contaminant and mycotoxin producer. Plant extracts and essential oils are a source of bioactive substances with antimicrobial potential that can be used instead of synthetic food preservatives. Spe...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/8/3437 |
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| author | Leonardo de Assunção Pinto Francisco Paiva Machado Ricardo Esteves Victor Moebus Farias Felipe Braz Nielsen Köptcke Eduardo Ricci-Junior Leandro Rocha Luiz Antonio Moura Keller |
| author_facet | Leonardo de Assunção Pinto Francisco Paiva Machado Ricardo Esteves Victor Moebus Farias Felipe Braz Nielsen Köptcke Eduardo Ricci-Junior Leandro Rocha Luiz Antonio Moura Keller |
| author_sort | Leonardo de Assunção Pinto |
| collection | DOAJ |
| description | The <i>Aspergillus</i> genus, the etiological agent of aspergillosis, is an important food contaminant and mycotoxin producer. Plant extracts and essential oils are a source of bioactive substances with antimicrobial potential that can be used instead of synthetic food preservatives. Species from the Lauraceae family and the <i>Ocotea</i> genus have been used as traditional medicinal herbs. Their essential oils can be nanoemulsified to enhance their stability and bioavailability and increase their use. Therefore, this study sought to prepare and characterize both nanoemulsion and essential oil from the <i>Ocotea indecora</i>’s leaves, a native and endemic species from the Mata Atlântica forest in Brazil, and evaluate the activity against <i>Aspergillus flavus</i> RC 2054, <i>Aspergillus parasiticus</i> NRRL 2999, and <i>Aspergillus westerdjikiae</i> NRRL 3174. The products were added to Sabouraud Dextrose Agar at concentrations of 256, 512, 1024, 2048, and 4096 µg/mL. The strains were inoculated and incubated for up to 96 h with two daily measurements. The results did not show fungicidal activity under these conditions. A fungistatic effect, however, was observed. The nanoemulsion decreased the fungistatic concentration of the essential oil more than ten times, mainly in <i>A. westerdjikiae</i>. There were no significant changes in aflatoxin production. |
| first_indexed | 2024-03-11T04:41:58Z |
| format | Article |
| id | doaj.art-e1100a65d5e34130826edd3fd0e84f6b |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T04:41:58Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e1100a65d5e34130826edd3fd0e84f6b2023-11-17T20:38:59ZengMDPI AGMolecules1420-30492023-04-01288343710.3390/molecules28083437Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from <i>Ocotea indecora</i> (Shott) Mez in <i>Aspergillus</i> SpeciesLeonardo de Assunção Pinto0Francisco Paiva Machado1Ricardo Esteves2Victor Moebus Farias3Felipe Braz Nielsen Köptcke4Eduardo Ricci-Junior5Leandro Rocha6Luiz Antonio Moura Keller7Programa de Pós-Graduação em Biotecnologia Vegetal e Bioprocessos, Centro de Ciências em Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro CEP 21941-590, BrazilPrograma de Pós-Graduação em Biotecnologia Vegetal e Bioprocessos, Centro de Ciências em Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro CEP 21941-590, BrazilPrograma de Pós-Graduação em Biotecnologia Vegetal e Bioprocessos, Centro de Ciências em Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro CEP 21941-590, BrazilPrograma de Pós-Graduação em Higiene Veterinária e Processamento Tecnológico de Produtos de Origem Animal, Faculdade de Veterinária, Universidade Federal Fluminense, Niterói, Rio de Janeiro CEP 24220-000, BrazilFaculdade de Farmácia, Universidade Federal Fluminense, Niterói, Rio de Janeiro CEP 24241-000, BrazilDepartamento de Medicamentos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro CEP 21941-902, BrazilFaculdade de Farmácia, Universidade Federal Fluminense, Niterói, Rio de Janeiro CEP 24241-000, BrazilDepartamento de Zootecnia e Desenvolvimento Agrosustentável, Faculdade de Veterinária, Universidade Federal Fluminense, Niterói, Rio de Janeiro CEP 24220-000, BrazilThe <i>Aspergillus</i> genus, the etiological agent of aspergillosis, is an important food contaminant and mycotoxin producer. Plant extracts and essential oils are a source of bioactive substances with antimicrobial potential that can be used instead of synthetic food preservatives. Species from the Lauraceae family and the <i>Ocotea</i> genus have been used as traditional medicinal herbs. Their essential oils can be nanoemulsified to enhance their stability and bioavailability and increase their use. Therefore, this study sought to prepare and characterize both nanoemulsion and essential oil from the <i>Ocotea indecora</i>’s leaves, a native and endemic species from the Mata Atlântica forest in Brazil, and evaluate the activity against <i>Aspergillus flavus</i> RC 2054, <i>Aspergillus parasiticus</i> NRRL 2999, and <i>Aspergillus westerdjikiae</i> NRRL 3174. The products were added to Sabouraud Dextrose Agar at concentrations of 256, 512, 1024, 2048, and 4096 µg/mL. The strains were inoculated and incubated for up to 96 h with two daily measurements. The results did not show fungicidal activity under these conditions. A fungistatic effect, however, was observed. The nanoemulsion decreased the fungistatic concentration of the essential oil more than ten times, mainly in <i>A. westerdjikiae</i>. There were no significant changes in aflatoxin production.https://www.mdpi.com/1420-3049/28/8/3437Flavi sectionfungistaticaflatoxinnatural productssesquirosefuransesquiterpenes |
| spellingShingle | Leonardo de Assunção Pinto Francisco Paiva Machado Ricardo Esteves Victor Moebus Farias Felipe Braz Nielsen Köptcke Eduardo Ricci-Junior Leandro Rocha Luiz Antonio Moura Keller Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from <i>Ocotea indecora</i> (Shott) Mez in <i>Aspergillus</i> Species Molecules Flavi section fungistatic aflatoxin natural products sesquirosefuran sesquiterpenes |
| title | Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from <i>Ocotea indecora</i> (Shott) Mez in <i>Aspergillus</i> Species |
| title_full | Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from <i>Ocotea indecora</i> (Shott) Mez in <i>Aspergillus</i> Species |
| title_fullStr | Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from <i>Ocotea indecora</i> (Shott) Mez in <i>Aspergillus</i> Species |
| title_full_unstemmed | Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from <i>Ocotea indecora</i> (Shott) Mez in <i>Aspergillus</i> Species |
| title_short | Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from <i>Ocotea indecora</i> (Shott) Mez in <i>Aspergillus</i> Species |
| title_sort | characterization and inhibitory effects of essential oil and nanoemulsion from i ocotea indecora i shott mez in i aspergillus i species |
| topic | Flavi section fungistatic aflatoxin natural products sesquirosefuran sesquiterpenes |
| url | https://www.mdpi.com/1420-3049/28/8/3437 |
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