Size-Exclusion Chromatography Combined with Ultrafiltration Efficiently Isolates Extracellular Vesicles from Human Blood Samples in Health and Disease

There is still a need for an efficient method for the isolation of extracellular vesicles (EVs) from human blood that provides a reliable yield with acceptable purity. Blood is a source of circulating EVs, but soluble proteins and lipoproteins hamper their concentration, isolation, and detection. Th...

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Bibliographic Details
Main Authors: Chiara Franco, Anna Ghirardello, Loris Bertazza, Michela Gasparotto, Elisabetta Zanatta, Luca Iaccarino, Hadi Valadi, Andrea Doria, Mariele Gatto
Format: Article
Language:English
Published: MDPI AG 2023-02-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/24/4/3663