Generation of Non-typeable Haemophilus influenzae Directed Gene Deletion Mutants

Directed deletion mutants in non-typeable Haemophilus influenzae can be made by allelic exchange of the target gene with an artificial DNA construct in which an antibiotic resistance cassette is placed between two ~1,000 bp DNA sequences that are identical to the 5' and 3' flanking r...

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Bibliographic Details
Main Author: Jeroen Langereis
Format: Article
Language:English
Published: Bio-protocol LLC 2014-03-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=1066&type=0