Generation of Non-typeable Haemophilus influenzae Directed Gene Deletion Mutants
Directed deletion mutants in non-typeable Haemophilus influenzae can be made by allelic exchange of the target gene with an artificial DNA construct in which an antibiotic resistance cassette is placed between two ~1,000 bp DNA sequences that are identical to the 5' and 3' flanking r...
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Format: | Article |
Language: | English |
Published: |
Bio-protocol LLC
2014-03-01
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Series: | Bio-Protocol |
Online Access: | https://bio-protocol.org/en/bpdetail?id=1066&type=0 |