Mechanism and Protective Effect of Smilax glabra Roxb on the Treatment of Heart Failure via Network Pharmacology Analysis and Vitro Verification
Smilax glabra Roxb (SGR) has been widely applied alone or in combination with other Chinese herbs in heart failure (HF), but its mechanism and protective effect have not been investigated. We aimed to explore the mechanism and protective effect of SGR on the treatment of HF. Network pharmacology ana...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.868680/full |
| _version_ | 1818530694957105152 |
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| author | Yingxin Long Zunjiang Li Chunxia Huang Zhongyu Lu Kuncheng Qiu Meixing He Zhijian Fang Banghan Ding Xiaohong Yuan Wei Zhu |
| author_facet | Yingxin Long Zunjiang Li Chunxia Huang Zhongyu Lu Kuncheng Qiu Meixing He Zhijian Fang Banghan Ding Xiaohong Yuan Wei Zhu |
| author_sort | Yingxin Long |
| collection | DOAJ |
| description | Smilax glabra Roxb (SGR) has been widely applied alone or in combination with other Chinese herbs in heart failure (HF), but its mechanism and protective effect have not been investigated. We aimed to explore the mechanism and protective effect of SGR on the treatment of HF. Network pharmacology analysis predicted that SGR was involved in the regulation of cell proliferation, oxidation–reduction process, apoptotic process, ERK1 and ERK2 cascade, MAPK cascade, etc. Its mechanism was mainly involved in the MAPK signaling pathway, calcium signaling pathway, cardiac muscle contraction, etc. Subsequently, SGR was proved to improve cellular viability, restore cellular morphology, suppress cellular and mitochondrial ROS production, improve H2O2-induced lysosome inhibition, attenuate mitochondrial dysfunction, and protect mitochondrial respiratory and energy metabolism in H9c2 cells. SGR activated the p38MAPK pathway by decreasing the mRNA expression of AKT, PP2A, NF-KB, PP2A, RAC1, and CDC42 and increasing the mRNA expression of Jun, IKK, and Sirt1. SGR also decreased the protein expression of ERK1, ERK2, JNK, Bax, and Caspase3 and increased the protein expression of p38MAPK and Bcl-2. In addition, Istidina at the highest degree was identified in SGR via the UHPLCLTQ-Orbitrap-MSn method, and it was suggested as anti-heart failure agents by targeting SRC with molecular docking analysis. In conclusion, SGR has a protective effect on HF through cellular and mitochondrial protection via multi-compounds and multi-targets, and its mechanism is involved in activating the p38 MAPK pathway. Istidina may be possible anti-HF agents by targeting SRC. |
| first_indexed | 2024-12-11T17:22:58Z |
| format | Article |
| id | doaj.art-e1b625ba230249cab178f5924af0b246 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-11T17:22:58Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-e1b625ba230249cab178f5924af0b2462022-12-22T00:57:04ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-05-011310.3389/fphar.2022.868680868680Mechanism and Protective Effect of Smilax glabra Roxb on the Treatment of Heart Failure via Network Pharmacology Analysis and Vitro VerificationYingxin Long0Zunjiang Li1Chunxia Huang2Zhongyu Lu3Kuncheng Qiu4Meixing He5Zhijian Fang6Banghan Ding7Xiaohong Yuan8Wei Zhu9The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Emergency, Panyu Hospital of Traditional Chinese Medicine, Guangzhou, ChinaGuangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, ChinaGuangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, ChinaGuangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, ChinaSmilax glabra Roxb (SGR) has been widely applied alone or in combination with other Chinese herbs in heart failure (HF), but its mechanism and protective effect have not been investigated. We aimed to explore the mechanism and protective effect of SGR on the treatment of HF. Network pharmacology analysis predicted that SGR was involved in the regulation of cell proliferation, oxidation–reduction process, apoptotic process, ERK1 and ERK2 cascade, MAPK cascade, etc. Its mechanism was mainly involved in the MAPK signaling pathway, calcium signaling pathway, cardiac muscle contraction, etc. Subsequently, SGR was proved to improve cellular viability, restore cellular morphology, suppress cellular and mitochondrial ROS production, improve H2O2-induced lysosome inhibition, attenuate mitochondrial dysfunction, and protect mitochondrial respiratory and energy metabolism in H9c2 cells. SGR activated the p38MAPK pathway by decreasing the mRNA expression of AKT, PP2A, NF-KB, PP2A, RAC1, and CDC42 and increasing the mRNA expression of Jun, IKK, and Sirt1. SGR also decreased the protein expression of ERK1, ERK2, JNK, Bax, and Caspase3 and increased the protein expression of p38MAPK and Bcl-2. In addition, Istidina at the highest degree was identified in SGR via the UHPLCLTQ-Orbitrap-MSn method, and it was suggested as anti-heart failure agents by targeting SRC with molecular docking analysis. In conclusion, SGR has a protective effect on HF through cellular and mitochondrial protection via multi-compounds and multi-targets, and its mechanism is involved in activating the p38 MAPK pathway. Istidina may be possible anti-HF agents by targeting SRC.https://www.frontiersin.org/articles/10.3389/fphar.2022.868680/fullSmilax glabra Roxbheart failuremitochondrial dysfunctionnetwork pharmacology analysisUHPLC-LTQ-Orbitrap-MSn method |
| spellingShingle | Yingxin Long Zunjiang Li Chunxia Huang Zhongyu Lu Kuncheng Qiu Meixing He Zhijian Fang Banghan Ding Xiaohong Yuan Wei Zhu Mechanism and Protective Effect of Smilax glabra Roxb on the Treatment of Heart Failure via Network Pharmacology Analysis and Vitro Verification Frontiers in Pharmacology Smilax glabra Roxb heart failure mitochondrial dysfunction network pharmacology analysis UHPLC-LTQ-Orbitrap-MSn method |
| title | Mechanism and Protective Effect of Smilax glabra Roxb on the Treatment of Heart Failure via Network Pharmacology Analysis and Vitro Verification |
| title_full | Mechanism and Protective Effect of Smilax glabra Roxb on the Treatment of Heart Failure via Network Pharmacology Analysis and Vitro Verification |
| title_fullStr | Mechanism and Protective Effect of Smilax glabra Roxb on the Treatment of Heart Failure via Network Pharmacology Analysis and Vitro Verification |
| title_full_unstemmed | Mechanism and Protective Effect of Smilax glabra Roxb on the Treatment of Heart Failure via Network Pharmacology Analysis and Vitro Verification |
| title_short | Mechanism and Protective Effect of Smilax glabra Roxb on the Treatment of Heart Failure via Network Pharmacology Analysis and Vitro Verification |
| title_sort | mechanism and protective effect of smilax glabra roxb on the treatment of heart failure via network pharmacology analysis and vitro verification |
| topic | Smilax glabra Roxb heart failure mitochondrial dysfunction network pharmacology analysis UHPLC-LTQ-Orbitrap-MSn method |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.868680/full |
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