Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins.
NK cells are important immune effectors for preventing microbial invasion and dissemination, through natural cytotoxicity and cytokine secretion. Bacillus anthracis spores can efficiently drive IFN-γ production by NK cells. The present study provides insights into the mechanisms of cytokine and cell...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC3257302?pdf=render |
| _version_ | 1818297890886385664 |
|---|---|
| author | Maria Klezovich-Bénard Jean-Philippe Corre Hélène Jusforgues-Saklani Daniel Fiole Nick Burjek Jean-Nicolas Tournier Pierre L Goossens |
| author_facet | Maria Klezovich-Bénard Jean-Philippe Corre Hélène Jusforgues-Saklani Daniel Fiole Nick Burjek Jean-Nicolas Tournier Pierre L Goossens |
| author_sort | Maria Klezovich-Bénard |
| collection | DOAJ |
| description | NK cells are important immune effectors for preventing microbial invasion and dissemination, through natural cytotoxicity and cytokine secretion. Bacillus anthracis spores can efficiently drive IFN-γ production by NK cells. The present study provides insights into the mechanisms of cytokine and cellular signaling that underlie the process of NK-cell activation by B. anthracis and the bacterial strategies to subvert and evade this response. Infection with non-toxigenic encapsulated B. anthracis induced recruitment of NK cells and macrophages into the mouse draining lymph node. Production of edema (ET) or lethal (LT) toxin during infection impaired this cellular recruitment. NK cell depletion led to accelerated systemic bacterial dissemination. IFN-γ production by NK cells in response to B. anthracis spores was: i) contact-dependent through RAE-1-NKG2D interaction with macrophages; ii) IL-12, IL-18, and IL-15-dependent, where IL-12 played a key role and regulated both NK cell and macrophage activation; and iii) required IL-18 for only an initial short time window. B. anthracis toxins subverted both NK cell essential functions. ET and LT disrupted IFN-γ production through different mechanisms. LT acted both on macrophages and NK cells, whereas ET mainly affected macrophages and did not alter NK cell capacity of IFN-γ secretion. In contrast, ET and LT inhibited the natural cytotoxicity function of NK cells, both in vitro and in vivo. The subverting action of ET thus led to dissociation in NK cell function and blocked natural cytotoxicity without affecting IFN-γ secretion. The high efficiency of this process stresses the impact that this toxin may exert in anthrax pathogenesis, and highlights a potential usefulness for controlling excessive cytotoxic responses in immunopathological diseases. Our findings therefore exemplify the delicate balance between bacterial stimulation and evasion strategies. This highlights the potential implication of the crosstalk between host innate defences and B. anthracis in initial anthrax control mechanisms. |
| first_indexed | 2024-12-13T04:26:38Z |
| format | Article |
| id | doaj.art-e1c1fb993f5a42baaf3497911735224d |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-13T04:26:38Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-e1c1fb993f5a42baaf3497911735224d2022-12-21T23:59:40ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742012-01-0181e100248110.1371/journal.ppat.1002481Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins.Maria Klezovich-BénardJean-Philippe CorreHélène Jusforgues-SaklaniDaniel FioleNick BurjekJean-Nicolas TournierPierre L GoossensNK cells are important immune effectors for preventing microbial invasion and dissemination, through natural cytotoxicity and cytokine secretion. Bacillus anthracis spores can efficiently drive IFN-γ production by NK cells. The present study provides insights into the mechanisms of cytokine and cellular signaling that underlie the process of NK-cell activation by B. anthracis and the bacterial strategies to subvert and evade this response. Infection with non-toxigenic encapsulated B. anthracis induced recruitment of NK cells and macrophages into the mouse draining lymph node. Production of edema (ET) or lethal (LT) toxin during infection impaired this cellular recruitment. NK cell depletion led to accelerated systemic bacterial dissemination. IFN-γ production by NK cells in response to B. anthracis spores was: i) contact-dependent through RAE-1-NKG2D interaction with macrophages; ii) IL-12, IL-18, and IL-15-dependent, where IL-12 played a key role and regulated both NK cell and macrophage activation; and iii) required IL-18 for only an initial short time window. B. anthracis toxins subverted both NK cell essential functions. ET and LT disrupted IFN-γ production through different mechanisms. LT acted both on macrophages and NK cells, whereas ET mainly affected macrophages and did not alter NK cell capacity of IFN-γ secretion. In contrast, ET and LT inhibited the natural cytotoxicity function of NK cells, both in vitro and in vivo. The subverting action of ET thus led to dissociation in NK cell function and blocked natural cytotoxicity without affecting IFN-γ secretion. The high efficiency of this process stresses the impact that this toxin may exert in anthrax pathogenesis, and highlights a potential usefulness for controlling excessive cytotoxic responses in immunopathological diseases. Our findings therefore exemplify the delicate balance between bacterial stimulation and evasion strategies. This highlights the potential implication of the crosstalk between host innate defences and B. anthracis in initial anthrax control mechanisms.http://europepmc.org/articles/PMC3257302?pdf=render |
| spellingShingle | Maria Klezovich-Bénard Jean-Philippe Corre Hélène Jusforgues-Saklani Daniel Fiole Nick Burjek Jean-Nicolas Tournier Pierre L Goossens Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. PLoS Pathogens |
| title | Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. |
| title_full | Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. |
| title_fullStr | Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. |
| title_full_unstemmed | Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. |
| title_short | Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. |
| title_sort | mechanisms of nk cell macrophage bacillus anthracis crosstalk a balance between stimulation by spores and differential disruption by toxins |
| url | http://europepmc.org/articles/PMC3257302?pdf=render |
| work_keys_str_mv | AT mariaklezovichbenard mechanismsofnkcellmacrophagebacillusanthraciscrosstalkabalancebetweenstimulationbysporesanddifferentialdisruptionbytoxins AT jeanphilippecorre mechanismsofnkcellmacrophagebacillusanthraciscrosstalkabalancebetweenstimulationbysporesanddifferentialdisruptionbytoxins AT helenejusforguessaklani mechanismsofnkcellmacrophagebacillusanthraciscrosstalkabalancebetweenstimulationbysporesanddifferentialdisruptionbytoxins AT danielfiole mechanismsofnkcellmacrophagebacillusanthraciscrosstalkabalancebetweenstimulationbysporesanddifferentialdisruptionbytoxins AT nickburjek mechanismsofnkcellmacrophagebacillusanthraciscrosstalkabalancebetweenstimulationbysporesanddifferentialdisruptionbytoxins AT jeannicolastournier mechanismsofnkcellmacrophagebacillusanthraciscrosstalkabalancebetweenstimulationbysporesanddifferentialdisruptionbytoxins AT pierrelgoossens mechanismsofnkcellmacrophagebacillusanthraciscrosstalkabalancebetweenstimulationbysporesanddifferentialdisruptionbytoxins |