Simplified PCR-Based Quantification of Proteins with DNA Aptamers and Methylcellulose as a Blocking Agent

Simplified PCR-Based Quantification of Proteins with DNA Aptamers and Methylcellulose as a Blocking Agent

Due to their unique three-dimensional structure, DNA or RNA oligonucleotide aptamers bind to various molecules with high affinity and specificity. Aptamers, alone or in combination with antibodies, can be used to sensitively quantify target molecules by quantitative real-time polymerase chain reacti...

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Bibliographic Details
Main Authors: Oleksij Redcenko, Magda Tumova, Petr Draber
Format: Article
Language:English
Published: MDPI AG 2023-12-01
Series:International Journal of Molecular Sciences
Subjects:
DNA aptamers
polymerase chain reaction
polypropylene wells
immunoglobulins
blocking agents
methylcellulose
Online Access:https://www.mdpi.com/1422-0067/25/1/347

Internet

https://www.mdpi.com/1422-0067/25/1/347

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