Uptake Assay for Radiolabeled Peptides in Yeast

We describe an assay for measuring the uptake of radioactive peptides into the yeast Saccharomyces cerevisiae. The methods presented here can be adapted to measure a variety of substrates transported into any bacterial or fungal cell via specific carrier-mediated systems.

Bibliographic Details
Main Authors: Melinda Hauser, Houjian Cai, Fred Naider, Jeffrey Becker
Format: Article
Language:English
Published: Bio-protocol LLC 2016-11-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=2026&type=0